Forthcoming articles

 


International Journal of Nano and Biomaterials

 

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International Journal of Nano and Biomaterials (22 papers in press)

 

Regular Issues

 

  • Synthesis of High Surface Area Boehmite and Alumina by Using Walnut Shell as Template   Order a copy of this article
    by Asghar Zamani, Ahmad Poursattar Marjani, Nasim Abdollahpour 
    Abstract: In the present study high surface area amorphous boehmite and γ-alumina were synthesized using walnut shell as template. This green, simple and useful synthetic protocol was based on the precipitation of aluminum hydroxide as the source of aluminum on biomass in an aqueous phase followed by calcination. The influence of walnut shell was evaluated by varying the calcination atmosphere. Materials were characterized using X-ray diffraction, fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, scanning electron microscopy and N2 adsorption/desorption porosimetry and the results exhibited high surface area for boehmite and γ-alumina. Furthermore, the pore size and surface area of these mesoporous materials can be adjusted by varying the experimental conditions. Additionally, boehmite was studied as the support of vanadium catalyst for the oxidation of alcohols by hydrogen peroxide. We have found that resulting V-loaded material act as an effective catalytic system for the oxidation of a wide range of alcohols in 1,4-dioxane. The catalyst can be recovered and reused four times without loss of activity.
    Keywords: green chemistry; biomass; walnut shell; alumina; boehmite; nanostructures; surface area; textural properties; catalysis; vanadium; oxidation; alcohols.

  • Microscopic study of the role of second nearest neighbour spin density wave coupling and electron hopping on superconducting gap   Order a copy of this article
    by Pratima Beura, Kamal Lochan Mohanta, Govinda Chandra Rout 
    Abstract: We report here a tight binding single band model Hamiltonian to study the role of spin density wave interaction and superconductivity. The Hamiltonian consists of nearest and next nearest neighbour electron hopping between the copper sides. The spin density wave interaction also includes nearest-neighbour and the next-nearest-neighbour spin interactions within a mean field approximation. BCS type superconducting interaction is considered taking d-wave pairing symmetry. By using Zubarevs Green function technique the Hamiltonian is solved. The antiferromagnetic and the superconducting gap equations are derived from the correlation functions and are solved self-consistently technique 100 × 100 grid points of the electron momentum. The evolution of these two order parameters are investigated by varying hole doping concentration, superconducting coupling, spin density wave coupling and second nearest neighbour electron hopping integrals.
    Keywords: high-temperature superconductor; d-wave pairing symmetry; spins density wave interaction.

  • Theoretical Study of Anisotropy in Orbital and Antiferromagnetic Spin Orderings in CMR Manganites   Order a copy of this article
    by Saswati Panda, D.D. Sahoo, G.C. Rout 
    Abstract: We report here a tight binding model study of the interplay of antiferromagnetic (AFM) spin and orbital orderings in the double exchange (DE) model for doped rare earth manganese oxides. In the kinetic energy interaction of conduction electrons, the first and the second nearest hopping integrals are considered. Kubo-Ohata type double exchange interaction is taken between the eg and t2g band electron spins of same site. A Heisenberg type antiferromagnetic spin-spin interaction is considered among the core band electrons, where both nearest and next nearest neighboring interactions are taken in the Heisenberg coupling. The presence of DE interaction induces antiferromagnetism in eg band. Due to the partial filling of eg band Jahn-Teller (JT) distortion is found in eg band. The JT distortion leads to anisotropy in the manganite systems. In the present model we have considered JT distortion as an extra mechanism. Finally the core t2g band electron interaction is considered in the model Hamiltonian. The model Hamiltonian is solved using Zubarevs Greens function technique. The temperature dependent lattice strain and transverse spin fluctuations are calculated from the electron Greens functions and the interplay between these order parameters are studied. Further, we have studied the in-plane anisotropy observed in the AFM spin fluctuation and its effect on the lattice strain.
    Keywords: Colossal magneto-resistance; Jahn-Teller effect; Electron correlationsrn.

  • The study of thermal properties of f-electron systems in the ferromagnetic state   Order a copy of this article
    by Asit Kumar Shadangi, Govinda Chandra Rout 
    Abstract: The rare earth and actinide series of compounds display anomalous physical properties below a characteristic low temperature with very high specific heat coefficient and high effective mass. Here we consider the periodic Anderson model with the repulsive electron-electron interaction within mean-field approximation leading to ferromagnetism in the system. We calculate conduction electron as well as f-electron Greens functions by using Zubarevs Greens function technique and calculate ferromagnetic magnetization numerically and self consistently. The thermal properties like the temperature dependent entropy, specific heat coefficient and electronic specific heat are calculated from the electron free-energy of the f-electron system and are computed numerically. The specific heat coefficient displays high value in heavy fermion state of the system where the position of the f-electron level is away from the Fermi level with the lower strength of hybridization between f and conduction electrons.
    Keywords: f-electron system; Entropy; Specific heat.

  • The tight-binding model study of the role of electron occupancy on the ferromagnetic gap in graphene-on-substrate   Order a copy of this article
    by Rashmirekha Swain, Sivabrata Sahu, G.C. Rout 
    Abstract: We propose here a theoretical model for graphene in its ferromagnetic phase. The Hamiltonian describes electron hoppings up-to-third-nearest neighbors for graphene-on-substrate. The sub-lattice Coulomb interactions within mean-field approach involves the total electron occupancy and ferromagnetic magnetizations. The temperature dependent ferromagnetic magnetization and hence the ferromagnetic gap are derived from the electron Greens functions and are solved self-consistently. The result shows that the magnitude of the ferromagnetic gap and the critical Coulomb interaction strongly depend on total electron occupancy. The critical Coulomb interaction decreases with increase of electron occupancy and the vice-versa.
    Keywords: Graphene; Ferromagnetic gap; Coulomb potential.

  • The theoretical study of the correlation between band filling and Coulomb interaction in the charge gap of graphene-on-substrate in paramagnetic limit   Order a copy of this article
    by Rudrashish Panda, Sivabrata Sahu, G.C. Rout 
    Abstract: The graphene having two dimensional honeycomb lattice is a zero band gap semiconductor where the conduction band and the valance band touch each other at the Dirac point. The two sub-lattices of the honeycomb lattice in pristine graphene are equivalent. When graphene is placed on a suitable substrate, the symmetry of the two sub lattices is broken leading to generation of a gap at the Fermi level. Under these conditions, the Coulomb interaction between the electrons in the two sub lattices play an important role in producing varieties of magnetic, non-magnetic and collective mode interactions. In the present communication, we propose a tight binding model Hamiltonian for graphene on a substrate with nearest neighbor hopping with dispersion in the total Brillouin zone in presence of symmetry breaking interaction due to substrate effect. The Coulomb interactions between the electrons in the two sub lattices are considered within mean-field approximation in the paramagnetic limit. The total Hamiltonian is solved by Zubarevs double time Greens function technique. The electron occupancies of the two sub-lattices are calculated from the correlation functions. Finally the expression for the temperature dependent charge gap i.e. ∆ ̅=U[〈n^a 〉-〈n^b 〉] is derived and calculated numerically. The evolution of the charge gap in graphene is investigated by varying the Coulomb interaction, electron occupancy and substrate induced gap. It is observed that the magnitude of the electron occupancy at A-site becomes larger than that at B-site throughout the temperature range indicating symmetry breaking of the two sub lattices of graphene.
    Keywords: Graphene; Paramagnetism; Coulomb Interaction; Magnetic gap.

  • Development of Self-Assembled Polygalacturonic Acid-Peptide Composites and their Interactions with Mesenchymal Stem Cells for Potential Applications in Tendon Tissue Engineering   Order a copy of this article
    by Grant Knoll, Harrison Pajovich, Steven Romanelli, Ipsita Banerjee 
    Abstract: We have developed a new biomimetic scaffold for potential applications in tendon tissue engineering. The scaffold template was synthesized by conjugating polygalacturonic acid with the dipeptide leucyl-leucine to mimic the leucine rich proteoglycans found in the extracellular matrix of tenocytes. To the template, Type I collagen and an elastin derived peptide were incorporated in order to form the final PG-Leu-Leu-Col-El scaffold. Results indicated the formation of gelatinous, fibrous scaffolds. DSC analysis showed phase changes that included crystallization and thermal melting due to re-organization of the scaffold components. Youngs Modulus was determined to be 832+/-2 MPa. Rheology studies showed that the scaffold maintained a constant G'/ G" ratio over a wide range of angular frequency. Cell studies with bone marrow derived mesenchymal stem cells indicated that the scaffolds promoted cell proliferation and formed three dimensional cell-scaffold matrices. This newly developed scaffold may open new opportunities for applications tissue engineering applications.
    Keywords: self-assembly; tissue engineering; composites; biocompatibility; microscale; peptide.

  • Theoretical Study Of Anisotropic Tunneling Conductance In Iron-based Orbitally Ordered Superconductors.   Order a copy of this article
    by Sushree Sangita Jena, S.K. Agarwalla, G.C. Rout 
    Abstract: We address here the role of Jahn-tellar (JT) distortion on the superconducting gap (SC) in Iron-based superconductors taking into account of the first and second nearest neighbor electron hoppings in the square lattice within one band model approach. The Greens functions are calculated by using Zubarevs Greens function technique. The temperature dependent superconducting gap and the lattice strain are calculated from the correlation functions of the corresponding Greens functions and are computed self-consistently. The temperature dependent gap equations show that the superconducting gap is enhanced with the decrease of second nearest neighbor hopping accompanied by the suppression of lattice strain near the superconducting transition temperature. The second nearest hopping introduces asymmetry in the tunneling conductance.
    Keywords: Iron-based superconductors; Jahn-Teller effect; scanning tunneling microscopy.

  • ELECTROSPINNING PRODUCTION OF PVA/CS/HEMA/nHA BIONANOCOMPOSITE   Order a copy of this article
    by Victor Manuel Castano 
    Abstract: PVA/CS/HEMA/nHA membranes were prepared by electrospinning at different voltages, to produce osteoinductive scaffolds that promote bone extracellular matrix production and in-situ remodeling. Infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) of samples obtained at 25 and 30 kV and 60 min spun, reveal that the presence of chitosan generates some malformations, and glutaraldehyde generates agglomerations in the threads. FTIR do not show significant changes in the functional groups, which indicate that these PVA/CS/HEMA/nHA scaffolds may represent a promising biomaterial for bone tissue engineering.
    Keywords: electrospinning; biopolymers; composite; membrane.

  • Hyaluronic Acid Carrier-Cell Interactions: A Tri-Culture Model of the Tumor Microenvironment to Study siRNA delivery under Flow Conditions   Order a copy of this article
    by Julio Manuel Rios De La Rosa, Nicola Tirelli, Annalisa Tirella 
    Abstract: CD44 is often overexpressed in solid tumors, rendering this protein a hot target in drug delivery. As CD44 is the main surface receptor of hyaluronic acid (HA), one of the most common therapeutic approaches consists of hijacking the cells mechanism of HA endocytosis to deliver active principles. This approach, however, presents two caveats: the poor understanding of HA-cell interactions and the ubiquitous expression of CD44 in other cell types, e.g. stromal cells. To predict the interaction of HA-decorated nanocarriers with CD44-expressing cells in the multicellular and complex tumor microenvironment, we have established a tri-culture, non-contact in vitro model (PANC-1 tumoral cells, HDF stromal cells, THP-1 macrophages) and quantified the delivery and kinetics of nanoparticle internalization (via flow cytometry), investigating the system in both static and dynamic culturing conditions. We report that HA-decorated nanocarriers are able to preferentially deliver siRNA to pancreatic cancer cells, interestingly even under flow/dynamic conditions.
    Keywords: Hyaluronic acid; CD44; tumor microenvironment; pancreatic cancer; macrophages; tumor-associated fibroblasts; nanoparticle; targeted drug delivery; siRNA.

  • Mechnosensitivity Response in Epithelial HT29 Cells on Titanium Dioxide Nanotube Arrays Surface via KRT8 Protein Expression   Order a copy of this article
    by Rabiatul Basria S M N Mydin, Srimala Sreekantan, Roshasnorlyza Hazan, Ekhlas Qaid Gazem, Mustafa Fadzil Farid Wajidi 
    Abstract: Titanium dioxide nanotube arrays (TNA) have been proposed as novel nano-surface modifications for biomaterial implants and nano-medicine applications. However, molecular mechanisms of cellTNA mechanosensitivity are not completely understood. Therefore, this study investigates the effect of cell-TNA mechanosensitivity activity via cytokeratin protein marker. Field Emission Scanning Electron Microscopy (FESEM) characterization were conducted on TNA with Epithelial-HT29 cells followed by analysis of cytokeratin protein marker using immunoflurescent staining and immunobloting techniques. FESEM results showed that cells grown on TNA exhibit enhanced cytoplasmic extension and spreading characteristics. Furthermore, the cells interaction with TNA nanosurface showed higher KRT8 expression indicate the clustering or aggregation of the cytoskeleton proteins. Findings suggested that Epithelial HT29 cells on TNA nano-topography might involve cytoskeleton mechanosensitivity response for the cellular adaptation activity.
    Keywords: Cellular Interaction with Nano-Topography; Titanium Dioxide Nanotube Arrays; Titania Nanotube Arrays; Cytoskeleton Reorganization; KRT8 protein; Cytokeratin-8; Bioengineered Nanomaterials; Mechanosensitivity.

Special Issue on: ISMA 2016 Smart Materials and Applications

  • Study on thermal field of a laser heated gemstone matrix by finite element method   Order a copy of this article
    by Radhakanta Dash, Pratima Mishra 
    Abstract: This paper presents the finite element modelling of the thermal field of a gemstone matrix by laser beam local heating. By using a continuous Gaussian beam of infrared laser a 3D temperature distribution in the gemstone material in the time domain is obtained. A tetrahedral mesh is used to solve the heat conduction equation in the entire 3D-structure. A transient thermal analysis of the interaction of the laser beam with the gemstone matrix reveals the temperature distribution profile throughout the volume of the material. The temperature profile of the computational model of the gemstone is in good agreement with the experimental observations.
    Keywords: gemstone matrix; alumina; sapphire; laser heating.
    DOI: 10.1504/IJNBM.2018.10016279
     
  • MM-wave double drift IMPATT diode: a comparison of experimental, theoretical and neural network approach   Order a copy of this article
    by P.R. Tripathy, S.K. Choudhury, S.P. Pati 
    Abstract: This paper presents the comparison of simulation and experimental output with the neural network results of different materials like Si and GaAs-based IMPATT diode at 94 GHz window frequency. The experimental results are nearly equal to simulation results and show a quantitative agreement between theoretical values and experimental output. The experimental results in terms of efficiency and RF power are almost 80-85% of the simulation results. Rest has 15-20% discrepancy between theory and experiment due to several causes like temperature, small-signal approach, parasitic effects, impedance matching problem and proper heat-sink arrangements. The developed neural network model is used to optimise performance of the diode by taking other wide band gap semiconductor material-based IMPATT diode. The model and the results will be very useful for applications in radar and guided missile technology.
    Keywords: silicon; gallium arsenide; double drift; IMPATT; RF power; efficiency; neural network.
    DOI: 10.1504/IJNBM.2018.10016278
     
  • Thin membrane with biomimetic hexagonal patterned surface for guided bone regeneration   Order a copy of this article
    by Anurag Satpathy, Rinkee Mohanty, Tapash Ranjan Rautray 
    Abstract: Polymeric membranes used as a barrier in guided tissue regeneration have undergone several modifications for improved structural, handling and biological properties. Biomaterial surface characteristics are critical in regulating cell function. Physicochemical signals from the substrate surfaces stimulate cell behaviour. This paper focuses on fabrication of biodegradable barrier membrane with hexagonal patterned surface for guided tissue regeneration. Hexagonal patterned surfaces were obtained during solvent casting of chitosan solution using a novel method. Obtained barrier membranes were analysed under stereo and electron microscopy and were assessed for their surface roughness. The hexagonal patterned surface barrier membranes had a significantly higher surface roughness (p < 0.001).
    Keywords: barrier membrane; chitosan film; hexagonal; patterned surface; surface roughness; guided bone regeneration; GBR.
    DOI: 10.1504/IJNBM.2018.10016280
     
  • Transport properties on MHD 3D ethylene glycol and water based colloidal suspensions (Al, CuO and SiC) nanoparticles: a numerical study   Order a copy of this article
    by Santosh Kumar Parida, Satyaranjan Mishra 
    Abstract: A comparative study has been made on thermal properties of magnetohydrodynamics (MHD) 3D ethylene glycol and water based colloidal suspension (Al, CuO and SiC) nanoparticles. Especially, fluid flow, heat and mass transfer characteristic for MHD three dimensional ethylene glycol based nanofluid is studied in the presence and absence of magnetic field. The involving nonlinear partial differential equations are reduced into ordinary differential equations by using similarity variables and then equations are solved numerically by Runge-Kutta method along with shooting technique. Nusselt number, Sherwood number and skin friction are calculated and their effect on the flow, heat and mass profiles are discussed graphically. It is interesting to observe that fluid velocity decreases with increase in Al-nanoparticles volume fraction whereas it increases for the nanoparticles CuO and SiC. Increase in the nanoparticles volume fraction increases the temperature both in presence and absence of magnetic parameter for all the three types of nanofluid under study.
    Keywords: magnetohydrodynamics; MHD; nanofluid; shooting technique; Runge-Kutta method.
    DOI: 10.1504/IJNBM.2018.10016281
     
  • Negative bias temperature instability in strained-Si p-MOSFETs   Order a copy of this article
    by Sanghamitra Das, T.P. Dash, Chinmay K. Maiti 
    Abstract: Strained-Si p-channel metal oxide semiconductor field effect transistors (MOSFETs) have become the performance boosters beyond 90 nm technology node. Reliability study of these devices is essential as only a few reports are available on this. In this work, we have explored the degradation mechanisms in these devices due to negative bias temperature instability (NBTI). Device simulation results have been calibrated with reported experimental data and a good agreement is observed. The reliability study of these devices has been performed using the two-stage model for defect creation. Study of the drain current degradation and comparison of threshold voltage shift after stressing between the strained-Si and Si channel p-MOSFETs have been performed. The threshold voltage degradation in strained-Si channel p-MOSFETs is found to be considerably higher than that in the bulk-Si devices due to higher fixed oxide charge and interface trap densities at the strained-Si/SiO2 interface.
    Keywords: negative bias temperature instability; NBTI; strained-Si channel p-MOSFET; threshold voltage degradation.
    DOI: 10.1504/IJNBM.2018.10016285
     
  • Preparation of TiO2 film on Ag and Al electrode for electrochemical bio-sensing application   Order a copy of this article
    by Nibedita Patro, Priyabrata Pattanaik, Sushanta Kumar Kamilla 
    Abstract: In this present, work, titanium dioxide (TiO2) deposited on Ag and Al electrodes were fabricated by sol-gel chemical wet and dry (CWD) method to detect urea. The formation of the polycrystalline TiO2 deposited on Ag and Al substrates were confirmed by X-ray diffraction (XRD) analysis. The XRD analyses of both the samples clearly indicated that some percentage of Ag and Al diffuses into the TiO2 matrix at its interface region. The nature of I-V characteristics showed by the TiO2 on Ag and Al wire electrodes are found to be Ohmic and is perfect to be used for electrochemical sensor or bio-sensor at very low potential value. The performance and response to various concentrations of urea solution and pH level were experimentally studied by using TiO2 coated on Ag and Al as working electrodes with respect to Ag as reference electrode.
    Keywords: titanium dioxide; TiO2; chemical wet and dry; CWD; X-ray diffraction analysis; XRD; ammonia sensor.
    DOI: 10.1504/IJNBM.2018.10016283
     
  • Structure and reaction dynamics of zirconium in the relativistic mean field model   Order a copy of this article
    by Mamina Panigrahi, Rabinarayan Panda 
    Abstract: In the framework of relativistic mean field theory using NL3, NL3*, NLSH, DD-PC1 and DD-ME2 parameter sets, the ground state binding energy, charge radius, quadrupole deformation parameter of three key isotopes of Zr, i.e., 90,92,94Zr has been studied. The results are compared with the experimental data and are found in reasonable agreement. The two-neutron separation energy and β-decay half-life of these isotopes are also evaluated. Total reaction and elastic differential scattering cross-section are also studied for these isotopes as projectile with 12C as target, using above parameter sets in conjunction with Glauber model and results are compared with the experimental data. All these investigations support the abundance of these three isotopes of zirconium.
    Keywords: relativistic mean field theory; Glauber model; total nuclear reaction cross-section; differential elastic scattering cross-section.
    DOI: 10.1504/IJNBM.2018.10016286
     

Special Issue on: Smart and Nano Materials Applications

  • Investigation on microstructures and phases of Fe-Ga alloy films deposited by magnetron sputtering   Order a copy of this article
    by Jianwu Yan, Ran Zhao, Yingpeng Cha, Qingpeng Li 
    Abstract: Galfenol (Fe-Ga alloy), as a new magnetostrictive materials, has potentially wide applications in magnetostrictive devices. In this work, Fe-Ga alloy thin films were prepared by slice-style target magnetron sputtering and investigations on microstructures and phases of Fe-Ga alloy films were made to explore the relationship of properties and microstructures. The results show that the component of alloy thin film is related to physical properties of the material itself as well as the area ratio of the patch and target. The phase formed in the films in is disorderly A2 phase with face-centered cubic structure. The films prepared by magnetron sputtering exist in the form of polycrystalline with <110> crystallographic texture perpendicular to the film plane. The structure shape of as-deposited specimens present a maze domain with different contrast and resolution and the magnetic domain decreased with the increase of the Ga content. With the increase of the Ga content, the magnetic domains become more and more irregular. Fe-Ga thin film morphology is related to the growth mode of the film. The microstructures of Fe-Ga alloy films can be controlled by magnetron sputtering technology.
    Keywords: Fe-Ga alloy films; magnetron sputtering; magnetic domain; A2 phase.

Special Issue on: Bio-Nano Materials and Drug Delivery Systems

  • Preparation of Micelle Supported Magnetic Hydroxylated Multi-walled Carbon Nanotubes Based Dispersive Solid-Phase Extraction for the Determination of PAHs in seawater   Order a copy of this article
    by Mingyu Wang, Shaojun ZHANG, Jiagan Li, Chengda Zhang, Shouwen Pang 
    Abstract: Measuring the impact of PAHs in seawater samples is often difficult due to the low concentrations in which they appear and the complexity of the sample matrix. Traditional methods for sample preparation such as liquid-liquid extraction and solid phase extraction require the use of excessive amounts of solvents and reagents, and sample handling. In this work, a micelle supported Fe3O4 magnetic nanoparticles decorated hydroxylated multi-walled carbon nanotubes material was synthesized. The material was facilely synthesized between Fe2+ and carbon nanotubes. The synthesized nanomaterial served as an excellent support for micelles, exhibiting high loading capacity and selectivity. The material could also be negatively charged by adjusting pH, exhibiting excellent extraction efficiency. The prepared material used in dispersive solid-phase extraction (DSPE) for investigation of polycyclic aromatic hydrocarbons (PAHs) from seawater for the first time. The application showed good response (R2 > 0.9981) in the range of 0.02 1.0
    Keywords: micelle; hydroxylated multi-walled carbon nanotubes; polycyclic aromatic hydrocarbons; dispersive solid-phase extraction.

  • Safety Evaluation of Nanomaterials in Fitness Equipment Based on Fuzzy Comprehensive Evaluation   Order a copy of this article
    by Rui Hua 
    Abstract: In order to evaluate the nanomaterials safety level in fitness equipment effectively, the fuzzy comprehensive evaluation method is applied in it. Firstly, the safety factors of nanomaterials in fitness equipment are discussed. Secondly, mathematical model and analysis procedure of fuzzy comprehensive evaluation method are studied. Thirdly, the simulation analysis is carried out for nanomaterials in fitness equipment for a province, and simulation results show that safety level of nanomaterials in fitness equipment for this province has serious injury on healthy and environment, which offers effective theoretical basis for establishing safety protection measures for nanomaterials in fitness equipment to promote development of national fitness.
    Keywords: Nanomaterials; Fitness equipment; Fuzzy comprehensive evalution; Safety evaluation.

Special Issue on: ICONAN 2017 Nanomedicine and Nanobiotechnology

  • Biogenic Nanosilver Inhibits Mycobacteria Using Nitrate Reductase Assay
    by Adebayo J. Bello, Luqman A. Adams, Nneka N. Onyejepu, Justice I. Igbinehi, Omoboyede I. Igbari, Joy Okpuzor 
    Abstract: Nanosilver was synthesized by bioreduction using aqueous extracts of Moringa oleifera (MO-AgNPs) leaf and Allium cepa bulb (AC-AgNPs) as reducing and capping agents. The nanosilver was characterized with UV/Vis, FT-IR, and SEM-EDX. Microbiological activity on 5 Mycobacteria strains: ATCC-35882, ATCC-25177, Mycobacterium other-than tuberculosis (MOTT), Mycobacterium fortuitum and Multi-Drug Resistance M. tuberculosis (MDR-MTB) were investigated using Nitrate Reductase Assay (NRA). Results obtained for UV/Vis gave absorption maxima at 430nm for MO-AgNPs and 440nm for AC-AgNPs. FT-IR showed functional groups associated with the biomolecular capping agents. SEM showed the nanosilver formed aggregates spherical and/or in oval shape with smooth surface. EDX spectra exhibited the presence of elemental silver. Minimum Inhibitory Concentration (MIC) study for both nanosilver against Mycobacteria indicated a higher inhibitory activity for MO-AgNPs over the AC-AgNPs. Here in, we have reported the first biosynthesized nanosilver activity against Mycobacteria using NRA. Nanosilver may therefore be developed as nanoaerosol for targeted and effective treatment of Mycobacteria infections.
    Keywords: Mycobacteria, tuberculosis, nanosilver, biosynthesis, Moringa oleifera, Allium cepa, nitrate reductase assay, MO-AgNPs, AC-AgNPs, Minimum Inhibitory Complex