Title: Cyclopropylamine modified plasma polymerised poly(methyl methacrylate) thin films for cell culture

Authors: Vincent Chan; Chuan Li; Ya-Hui Tsai; Y.H. Tseng; Yun Chen

Addresses: Department of Chemical Engineering, Khalifa University, P.O. Box 127788, Abu Dhabi, UAE ' Department of Biomedical Engineering, National Yang Ming University Taipei, 155 Sec. 2 Linong St., Beitou, Taipei, 112, Taiwan; Department of Mechanical Engineering, National Central University, 300 Zhongda Rd., Zhongli, Taoyuan, 320, Taiwan ' Department of Surgery, Far Eastern Memorial Hospital, 21 Sec. 2 Nanya S. Rd., Banqiao, New Taipei City, 220, Taiwan ' Department of Biomedical Engineering, National Yang Ming University Taipei, 155 Sec. 2 Linong St., Beitou, Taipei, 112, Taiwan ' Department of Surgery, Far Eastern Memorial Hospital, 21 Sec. 2 Nanya S. Rd., Banqiao, New Taipei City, 220, Taiwan

Abstract: Cyclopropylamine (CPA) is a biochemical active, volatile low carbon polymer. It usually is rendered as a compound, for instance the N-substituted cyclopropylamine, to synthesise many antidepressants or anticancer pharmaceuticals. On the other hand, poly(methyl methacrylate) (PMMA) commonly known as acrylic is a transparent thermoplastic polymer. Because of its nontoxic, stable physical and chemical properties, and because it is inexpensive and easy to processed, PMMA is widely used as structural and adhesive material. In this study, we fabricated CPA-PMMA thin films by plasma polymerisation and investigated the microstructure, surface morphology, optical properties and wettability of films. The plasma polymerisation was conducted in a vacuum chamber powered by a radio frequency supply. The composition of mixed monomers was controlled by the ratio of partial pressure of monomer gases. The deposited films were subjected to the following measurements: surface profiler for the average thickness and deposition rate, Fourier transform infrared (FTIR) spectrometer for the vibrational modes of microstructures; contact angle for the wettability, and UV-Vis-NIR spectrometer for the optical transparency and absorption. Finally these films were prepared for cell culture and the host was chosen to be 3T3 fibroblasts. The culture was assessed by MTT assays to evaluate the cellular viability in culture media and directly on films. Results from cell culture showed that all films are nontoxic and capable of supporting the growth of 3T3 fibroblasts.

Keywords: poly(methyl methacrylate); PMMA; cyclopropylamine; plasma polymerisation; 3T3 fibroblast; MTT assay.

DOI: 10.1504/IJNT.2017.087781

International Journal of Nanotechnology, 2017 Vol.14 No.12, pp.1045 - 1065

Published online: 23 Sep 2017 *

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