Title: In vitro assessment on immunomodulatory effects of Lactobacillus collinoides

Authors: A. Ashretha Latha; U. Aarthy; R.B. Narayanan

Addresses: Centre for Biotechnology, Anna University, Chennai-25, India ' Centre for Biotechnology, Anna University, Chennai-25, India ' Centre for Biotechnology, Anna University, Chennai-25, India

Abstract: Lymphoproliferation, cytokine gene expression and apoptosis was studied in the peripheral blood mononuclear cells (PBMCs) of ten normal individuals stimulated in vitro with live and heat killed L. collinoides. Live L. collinoides induced higher levels of lymphoproliferation compared to heat killed preparation. Both live and heat killed L. collinoides stimulated cultures exhibited enhanced IL-2 and IFN-γ gene expression to the same degree compared to the expression of IL-4 and IL-10 genes. Nitric oxide levels were elevated in the culture supernatants of live L. collinoides stimulated cultures compared to the heat killed ones. Neither of the L. collinoides preparations induced apoptosis in the PBMCs. Antibodies to L. collinoides were not detected in the sera of these individuals. These findings suggest that Lactobacillus collinoides promotes preferentially Th1 type immune response.

Keywords: lymphoproliferation; cytokine gene expression; apoptosis; L. collinoides; nitric oxide; peripheral blood mononuclear cells; PBMCs; probiotics; in vitro assessment; immunomodulatory effects; Lactobacillus collinoides; immune response.

DOI: 10.1504/IJMEI.2015.070119

International Journal of Medical Engineering and Informatics, 2015 Vol.7 No.3, pp.222 - 232

Received: 23 Oct 2013
Accepted: 19 May 2014
Published online: 27 Jun 2015 *

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