Title: Influence of X-ray and/or CpG-DNA induced oxidative stress on adaptive response in human lymphocytes

Authors: Marina S. Konkova, Aleksei V. Ermakov, Liudmila V. Efremova, Svetlana V. Kostyuk, Natalya N. Veiko

Addresses: Research Centre for Medical Genetics, Russian Academy of Medical Sciences, Moskvorechye Street, 1, 115478, Moscow, Russia. ' Research Centre for Medical Genetics, Russian Academy of Medical Sciences, Moskvorechye Street, 1, 115478, Moscow, Russia. ' Research Centre for Medical Genetics, Russian Academy of Medical Sciences, Moskvorechye Street, 1, 115478, Moscow, Russia. ' Research Centre for Medical Genetics, Russian Academy of Medical Sciences, Moskvorechye Street, 1, 115478, Moscow, Russia. ' Research Centre for Medical Genetics, Russian Academy of Medical Sciences, Moskvorechye Street, 1, 115478, Moscow, Russia

Abstract: Peripheral blood lymphocytes of apparently healthy donors were exposed to low-dose ionising radiation and/or human-genome CpG-DNA fragments accumulating in total extracellular DNA. Both factors administered separately appeared to induce transposition of the 1q12 loci of homologous chromosomes in lymphocytes from the pericentromeric region towards the centre of the nucleus; they were also shown to activate rRNA synthesis, to stimulate activation of caspase-3 activity and to increase amounts of the TLR9 and MyD88 mRNAs. However, upon simultaneous exposure to X-ray radiation and CpG-DNA, all the reactions mentioned above failed to develop in the cells. Practical aspects of the revealed phenomenon are discussed in this paper.

Keywords: adaptive response; bystander effect; peripheral blood lymphocytes; TLR9; MyD88; extracellular DNA; CpG-DNA; low radiation; ionising radiation; low-dose radiation; X-ray radiation; oxidative stress.

DOI: 10.1504/IJLR.2010.037667

International Journal of Low Radiation, 2010 Vol.7 No.6, pp.446 - 452

Available online: 23 Dec 2010 *

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