Title: The response of peripheral blood mononuclear cells to shape memory alloys

Authors: Rebeka Rudolf, Kambiz Mehrabi, Albert C. Kneissl, Mihael Bruncko, Ivan Anzel, Sergej Tomic, Tanja Dzopalic, Miodrag Colic

Addresses: Faculty of Mechanical Engineering, University of Maribor, Smetanova 17 SI-2000 Maribor, Slovenia; Zlatarna Celje d.d., Kersnikova 19, SI-3000 Celje, Slovenia. ' Department of Physical Metallurgy and Materials Testing, University of Leoben, Franz-Josef-Strasse 18, 8700 Leoben, Austria. ' Department of Physical Metallurgy and Materials Testing, University of Leoben, Franz-Josef-Strasse 18, 8700 Leoben, Austria. ' Faculty of Mechanical Engineering, University of Maribor, Smetanova 17 SI-2000 Maribor, Slovenia. ' Faculty of Mechanical Engineering, University of Maribor, Smetanova 17 SI-2000 Maribor, Slovenia. ' Institute of Medical Research, Military Medical Academy, Crnotravska 17 11002 Belgrade, Serbia. ' Institute of Medical Research, Military Medical Academy, Crnotravska 17 11002 Belgrade, Serbia; Medical Faculty Nis, University of Nis, Bulevar dr Zorana Dindica, 81 18000 Nis, Serbia. ' Institute of Medical Research, Military Medical Academy, Crnotravska 17 11002 Belgrade, Serbia; Medical Faculty Nis, University of Nis, Bulevar dr Zorana Dindica, 81 18000 Nis, Serbia

Abstract: The aim of this work was to study the response of human peripheral blood mononuclear cells (PB-MNCs) to Cu-Al-Ni and Ni-Ti shape memory alloys (SMAs). The alloys were prepared as rapidly solidified thin ribbons via melt spinning. It has been shown that the ribbons were non cytotoxic for PB-MNCs as determined by the viability and apoptosis assay. In 18 cultures out of 20 cultures of individual donor PB-MNCs cultivated with Cu-Al-Ni SMAs, no significant changes in the production of Th1 (IFN-γ), Th2 (IL-5 and IL-10) and proinflammatory (IL-1ß, TNF-α, IL-6 and IL-8) cytokines were detected compared to controls. In two cultures out of 20, a significant increase in the production of Th1 and proinflammatory cytokines was observed. Microstructural analyses confirmed the formation of thin oxide on the surface of both SMA ribbons.

Keywords: shape memory alloys; SMAs; peripheral blood mononuclear cells; cytotoxicity; cytokines; microstructural analysis; human blood cells; microstructure.

DOI: 10.1504/IJIS.2010.034903

International Journal of Immunological Studies, 2010 Vol.1 No.2, pp.214 - 228

Published online: 30 Aug 2010 *

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