Authors: Stephen J. Sowerby, George B. Petersen
Addresses: Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand. ' Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand
Abstract: We propose that a DNA entropic trap constructed from a single resizable nanopore could be tuned in real time to capture a single-stranded polynucleotide. With a nanopore of sufficient discriminatory capability, the trapped polynucleotide will be immobilised within the corrugational trough of an inter-nucleotide separation and the prolonged dwell time will enable scrutiny of the nucleotide composition by extended measurement of the resistive pulse signal. The nanopore will be tuned to facilitate single nucleotide translocation, allowing for the processive examination of consecutive nucleotides.
Keywords: Brownian; Coulter; ratchet; sensor; stochastic; nanotechnology; single molecule DNA; DNA sequencing; resizable nanopores; DNA entropic trap; polynucleotides; single nucleotide translocation.
International Journal of Nanotechnology, 2009 Vol.6 No.3/4, pp.398 - 407
Published online: 03 Feb 2009 *Full-text access for editors Access for subscribers Purchase this article Comment on this article