Mechanical characterisation of human cartilage cells by AFM nanoindentation under different loading frequencies
by Kexiang Hu; Limin Sun; Qingkang Wang
International Journal of Nanomanufacturing (IJNM), Vol. 10, No. 5/6, 2014

Abstract: In this paper, we employ the AFM tip indentation and Hertzian contact mechanics model to compute Young's modulus and the adhesion force of cartilage cells in different environments. The indentation is implemented under different loading frequencies to obtain the relationship between loading frequency and Young's modulus of both living and dead cartilage cells. The results show that the stiffness of the cell membrane is related to the loading frequency. Under the constant loading force, the indented depth decreases with the increase of frequency. The less the indented depth is, the stiffer the cell membrane will be, therefore, the larger the Young's modulus will be. This study also shows the adhesive force of living cartilage cells lies within the range from 150 nN to 350 nN, while the dead ones have the adhesive force between 30 nN and 90 nN.

Online publication date: Sat, 20-Dec-2014

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Nanomanufacturing (IJNM):
Login with your Inderscience username and password:

    Username:        Password:         

Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email