Application of silicon nanowire for detection and quantitative analysis of alpha-fetoprotein biomarker
by Van Binh Pham; Thi Thanh Tuyen Le; Thanh Nhat Khoa Phan; Tien Thang Nguyen; Chien Mau Dang
International Journal of Nanotechnology (IJNT), Vol. 15, No. 1/2/3, 2018

Abstract: Semiconductor silicon nanowires (SiNWs) have shown great potential in detection and quantitative analysis of human disease biomarkers. Compared to current methods such as fluorescence diagnosis, enzyme linked immunosorbent assays (ELISA) or western blot, the label-free technique using SiNWs as sensors allows fast, efficient and inexpensive detection and quantification of biomarkers. In this research, SiNWs surfaces were modified by antibody of alpha-fetoprotein (AFP), a well-known biomarker of liver cancer. This antibody was immobilised to facilitate the specific capture of AFP. The efficiencies of surface modification and antibody immobilisation were estimated by contact angle measurement, fluorescence microscopy, chromogenic reaction, ultraviolet-visible (UV-Vis) spectroscopy and electrical characterisation of the SiNWs after every surface modification step. A calibration curve between AFP concentration and SiNWs' electrical response was built, from which any other sample with arbitrary concentration of AFP can be quantitatively diagnosed with the SiNWs.

Online publication date: Tue, 30-Jan-2018

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

 
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Nanotechnology (IJNT):
Login with your Inderscience username and password:

Username:        Password:          Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com