Nanoapertures for AFM-based single-molecule force spectroscopy Online publication date: Sat, 27-Apr-2013
by Stephan F. Heucke; Elias M. Puchner; Stefan W. Stahl; Alexander W. Holleitner; Hermann E. Gaub; Philip Tinnefeld
International Journal of Nanotechnology (IJNT), Vol. 10, No. 5/6/7, 2013
Abstract: Simultaneous single-molecule force spectroscopy and microfluorescence binding measurements are often hampered by background fluorescence from the bulk. Zero-Mode Waveguides (ZMW) restrict the excited volume but require a special design, which allows the tip of the force probing cantilever to protrude into the nanoaperture. Here, we describe the design and fabrication of such ZMW and report the first experiments where binding of fluorescent adenosine triphosphate to the force-activated enzyme titin kinase was measured while the enzyme was subjected to mechanical forces.
Online publication date: Sat, 27-Apr-2013
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Nanotechnology (IJNT):
Login with your Inderscience username and password:
Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.
If you still need assistance, please email firstname.lastname@example.org