Single-particle analysis and electron tomography
by Kenji Iwasaki
International Journal of Nanotechnology (IJNT), Vol. 3, No. 4, 2006

Abstract: Both single-particle analysis and electron tomography are special tools for the structural analysis of biological components by transmission electron microscopy. Single-particle analysis enables us to determine the structures of relatively large proteins and macromolecular complexes without crystallisation. Electron tomography is often used to visualise organelles and subcellular structures at molecular resolution. Recently, the scope of electron tomography has been widened due to the introduction of an automatic data collection system, cryo-electron microscopy, and improved sample preparation techniques. Application of electron tomography to single-particle analysis will be capable of analysing dynamics of large proteins. The techniques described in this review are expected to bridge the gap between the atomic resolution structures of protein molecules and cellular structures obtained by light microscopy.

Online publication date: Fri, 20-Oct-2006

The full text of this article is only available to individual subscribers or to users at subscribing institutions.

 
Existing subscribers:
Go to Inderscience Online Journals to access the Full Text of this article.

Pay per view:
If you are not a subscriber and you just want to read the full contents of this article, buy online access here.

Complimentary Subscribers, Editors or Members of the Editorial Board of the International Journal of Nanotechnology (IJNT):
Login with your Inderscience username and password:

Username:        Password:          Forgotten your password?

Want to subscribe?
A subscription gives you complete access to all articles in the current issue, as well as to all articles in the previous three years (where applicable). See our Orders page to subscribe.

If you still need assistance, please email subs@inderscience.com