Title: Circular electrodes stepping manipulation platform for A549 cancer cell detection

Authors: Do Quang Loc; Nguyen Thu Trang; Vo Thi Thuong Lan; Hoang Thi My Nhung; Tran Thi Thuy Ha; Le Van Chieu; Nguyen Ngoc Viet; Chun-Ping Jen; Bui Thanh Tung; Trinh Chu Duc

Addresses: Faculty of Physics, University of Science, Vietnam National University, 334, Nguyen Trai, Hanoi, 10000, Vietnam ' Faculty of Biology, University of Science, Vietnam National University, 334, Nguyen Trai, Hanoi, 10000, Vietnam ' Faculty of Biology, University of Science, Vietnam National University, 334, Nguyen Trai, Hanoi, 10000, Vietnam ' Faculty of Biology, University of Science, Vietnam National University, 334, Nguyen Trai, Hanoi, 10000, Vietnam ' Faculty of Electronics Engineering, Posts and Telecommunications Institute of Technology, Km10, Nguyen Trai Rd., Ha Dong Dist., Hanoi, 10000, Vietnam ' Faculty of Environmental Science, University of Science, Vietnam National University, 334, Nguyen Trai, Thanh Xuan, Hanoi, 10000, Vietnam ' College of Engineering, National Chung Cheng University, No.168, Sec. 1, University Rd., Min-Hsiung, Chia-yi County 621, Taiwan ' College of Engineering, National Chung Cheng University, No.168, Sec. 1, University Rd., Min-Hsiung, Chia-yi County 621, Taiwan ' Faculty of Electronics and Telecommunication, University of Engineering and Technology, Vietnam National University, 144, Xuan Thuy, Cau Giay, Hanoi, 10000, Vietnam ' Faculty of Electronics and Telecommunication, University of Engineering and Technology, Vietnam National University, 144, Xuan Thuy, Cau Giay, Hanoi, 10000, Vietnam

Abstract: In this paper, a novel design of an impedance sensor integrated on stepping dielectrophoresis manipulation platform for biological cells detection is presented. The platform consists of fluidic working chamber and manipulating/sensing gold electrodes. Gold electrodes are patterned on glass substrate with an insulating layer covered to avoid the direct contact between metal electrodes and electrolyte. The outer electrodes implement biological cell manipulating function while the inner electrodes construct sensing electrodes pair which are connected to an impedance analyser to detect the appearance of biological cells on the sensing area. In this work, several experiments have been implemented to investigate the performance of A549 cell line trapping ability based on aptamer immobilisation on gold surface method with different concentrations of aptamer solution. Impedance spectroscopy responses of the proposed sensor with frequency ranges from 10 kHz to 1.0 MHz are examined. The impedance spectroscopy shows an impedance change of up to approximately 2500 Ω at 200 kHz when 16 A549 cells appear in the sensing area.

Keywords: impedance spectroscopy; CTCs; circulating tumour cells; aptamer; A549 cancer cell.

DOI: 10.1504/IJNT.2018.099936

International Journal of Nanotechnology, 2018 Vol.15 No.11/12, pp.983 - 997

Available online: 24 May 2019 *

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