Title: An in silico approach for construction of a chimeric protein, targeting virulence factors of Shigella spp.

Authors: Emad Kordbacheh; Shahram Nazarian; Amin Farhang

Addresses: Faculty of Science, Department of Biology, Imam Hossein University, Tehran, 1651255117, Iran ' Faculty of Science, Department of Biology, Imam Hossein University, Tehran, 1651255117, Iran ' Faculty of Science, Department of Biology, Imam Hossein University, Tehran, 1651255117, Iran

Abstract: Shigellosis is a high burden gastrointestinal disease with an increased frequency of antibiotic resistance. Type III secretion apparatus (T3SA) are conserved among different species of Shigella; and IpaD, IpaB, and IcsA proteins participate in its function. Studies indicate shiga toxin as a virulence factor has a fundamental role in hemorrhagic colitis. Bioinformatics tools were recruited for aiding this purpose. In the level of the nucleosome, sequences choosing and optimising and in the phase of the transcriptome, some prediction in associate with mRNA form, also in step of the proteome, physicochemical parameter, best stability, first to third structures and model validation were some prediction performed in assistance with in silico servers. Moreover, estimating antigenic and allergenic propensity, subcellular localisation and protein function were accomplished by bioinformatics software. Finally, these results would be beneficial in an animal model purpose for development of a pervasive candidate immunogen against Shigella spp.

Keywords: Shigella species; bioinformatics; subunit vaccine; virulence factors.

DOI: 10.1504/IJCBDD.2018.096127

International Journal of Computational Biology and Drug Design, 2018 Vol.11 No.4, pp.310 - 327

Received: 06 Sep 2017
Accepted: 18 Dec 2017
Published online: 13 Nov 2018 *

Full-text access for editors Full-text access for subscribers Purchase this article Comment on this article