Title: Elaboration of a bacterial cellulose matrix for the immobilisation of Escherichia coli cells

Authors: T.I. Gromovykh; N.B. Feldman; O.A. Tikhonova; S.V. Lutsenko; P.S. Timashev; K.N. Bardakova; S.N. Churbanov; О.I. Kiselyova; М.N. Kraeva; А.S. Grinevich

Addresses: Department of Biotechnology, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia ' Department of Biotechnology, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia ' Department of Biotechnology, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia ' Department of Biotechnology, Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia ' Department of Advanced Biomaterials, Institute for Regenerative Medicine, Federal State Autonomous Educational Institution of Higher Education, I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia; Federal Research Centre "Crystallography and Photonics", The Russian Academy of Sciences, Institute of Photonic Technologies, Pionerskaya St. 2, Moscow, Troitsk, 142190, Russia ' Department of Advanced Biomaterials, Institute for Regenerative Medicine, Federal State Autonomous Educational Institution of Higher Education, I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia; Federal Research Centre "Crystallography and Photonics", The Russian Academy of Sciences, Institute of Photonic Technologies, Pionerskaya St. 2, Moscow, Troitsk, 142190, Russia ' Department of Advanced Biomaterials, Institute for Regenerative Medicine, Federal State Autonomous Educational Institution of Higher Education, I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Bolshaya Pirogovskaya St., Moscow, 119991, Russia; Federal Research Centre "Crystallography and Photonics", The Russian Academy of Sciences, Institute of Photonic Technologies, Pionerskaya St. 2, Moscow, Troitsk, 142190, Russia ' Department of Physics, Moscow State University named after M.V. Lomonosov, Leninskie Gory, Moscow, 119991, Russia ' Federal State Budgetary University "Russian Cancer Research Center named after N.N. Blokhin" of the Ministry of Health of Russia, Kashirskoe sh. 24 bld. 2, 115409, Moscow, Russia ' Federal State Budgetary University "Russian Cancer Research Center named after N.N. Blokhin" of the Ministry of Health of Russia, Kashirskoe sh. 24 bld. 2, 115409, Moscow, Russia

Abstract: This paper is concerned with the studies of a natural nanomaterial which is bacterial cellulose synthesised by Gluconacetobacter hansenii producer strain. It covers different fields of bacterial cellulose use, including medicine. The research has proved that bacterial cellulose matrices with immobilised cells have high potential as immobilisers of cells, including making probiotics of prolonged action. The matrices consisted of bacterial cellulose films were prepared by static cultivation of G. hansenii GH-1/2008 strain in the liquid medium. We have developed methods of washing out end toxins and producer cells of the films in the solutions of sodium bicarbonate, sodium dodecyl sulphate, and sodium hydroxide. The LAL-test has revealed that washing the films with sodium dodecyl sulphate is more efficient. By means of electron scanning and atomic force microscopy (AFM), we have determined that bacterial cellulose matrices have a layered structure, smooth surface, and adhesion of E. coli test strain cells. The adhesive capacity, the energy of adhesion and contact angle is higher for 50 um thick films than for 20 um thick ones. The bacterial cellulose matrices obtained by the biosynthesis of G. hansenii strain can be recommended for the immobilisation of different producer cells.

Keywords: bacterial cellulose; a nanomaterial; Gluconacetobacter hansenii bacteria; adhesion; Escherichia coli.

DOI: 10.1504/IJNT.2018.094784

International Journal of Nanotechnology, 2018 Vol.15 No.4/5, pp.288 - 300

Published online: 15 Sep 2018 *

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