Title: Influence of chondroitin 4-sulphate on properties and cell behaviour of collagen hydrogel

Authors: Masrina Mohd Nadzir; Lau Sin Mun; Farah Dhaniyah Anour; Shiplu Roy Chowdhury; Mohd Fauzi Mh Busra

Addresses: School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia ' School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia ' School of Chemical Engineering, Engineering Campus, Universiti Sains Malaysia, 14300 Nibong Tebal, Penang, Malaysia ' Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia ' Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Kuala Lumpur, Malaysia

Abstract: In this research, the fabrication of collagen/chondroitin 4-sulphate (C4S) hydrogel scaffold for biomedical applications was studied. The influence of C4S content on physical, mechanical, and biological properties of scaffold were investigated. It was found that high C4S content significantly increased the swelling capacity of scaffold. Furthermore, thermal stability was higher by 5°C in the presence of C4S. Human dermal fibroblasts were cultured and bioactivity of collagen/C4S hydrogel scaffolds was assayed. Although affinity of cell attachment was reduced on hydrogel scaffold with C4S, cells were able to spread well and displayed stretched morphology. Cell viability assay was performed to evaluate cell proliferation on collagen/C4S hydrogel scaffold. The results confirmed the inclusion of C4S to collagen improved the properties of hydrogel scaffold without affecting the growth rate of cells.

Keywords: hydrogel scaffold; collagen; chondroitin 4-sulphate; characterisation; in vitro; biomaterials.

DOI: 10.1504/IJNBM.2018.094252

International Journal of Nano and Biomaterials, 2018 Vol.7 No.3, pp.242 - 250

Received: 30 May 2017
Accepted: 21 Nov 2017
Published online: 23 Aug 2018 *

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