Title: Collagen/chitosan hybrid 3D-scaffolds as potential biomaterials for tissue engineering

Authors: Hilary Ureña-Saborio; Emilia Alfaro-Viquez; Daniel Esquivel-Alvarado; Marianelly Esquivel; Sergio Madrigal-Carballo

Addresses: National Center for Biotechnological Innovations (CENIBiot), CeNAT-CONARE, 1174-1200 San Jose, Costa Rica ' National Center for Biotechnological Innovations (CENIBiot), CeNAT-CONARE, 1174-1200 San Jose, Costa Rica ' National Center for Biotechnological Innovations (CENIBiot), CeNAT-CONARE, 1174-1200 San Jose, Costa Rica ' Polymers Research Laboratory (POLIUNA), School of Chemistry, National University, 86-3000 Heredia, Costa Rica ' National Center for Biotechnological Innovations (CENIBiot), CeNAT-CONARE, 1174-1200 San Jose, Costa Rica; Polymers Research Laboratory (POLIUNA), School of Chemistry, National University, 86-3000 Heredia, Costa Rica

Abstract: Chitosan (CHT) has been reported to be biocompatible, bio-absorbable and particularly, is considered a good wound-healing accelerator. On the other hand, collagen (CGN) is one of the most widely used matrix biomaterial in tissue engineering. Highly porous CGN single 3D-scaffolds have been used to support in vitro growth of many types of tissues. We fabricated hybrid 3D-scaffold biomaterials by mixing CHT from native shrimp waste streams and CGN from tilapia aquaculture waste by-products, applying previously developed methodologies involving solvent casting and freeze-drying. CGN/CHT hybrid 3D-scaffolds were characterised according to its water uptake capacity, mechanical properties, thermal behaviour (TGA), and morphology (SEM). Hybrid 3D-scaffolds showed improved stability, greater porosity, increased thermal stability and mechanical properties, as well higher biodegradation as compared to single 3D-scaffolds. Cell culture incubation with adipose tissue-derived stem cell (ADSC) and SEM imaging showed that CGN/CHT hybrid 3D-scaffolds allowed ADSC adhering, spreading, and growing in vitro.

Keywords: collagen; chitosan; scaffolds; tissue engineering.

DOI: 10.1504/IJNBM.2018.094240

International Journal of Nano and Biomaterials, 2018 Vol.7 No.3, pp.163 - 175

Available online: 23 Aug 2018

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