Title: Simultaneous application arsenic oxidising bacteria and biochar for the reclamation of arsenic contaminated soil

Authors: Santosh Kumar Karn; Xiangliang Pan

Addresses: State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, Urumqi, 830011 Xinjiang, China; Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, Urumqi, 830011 Xinjiang, China; Department of Biochemistry and Biotechnology, Sardar Bhagwan Singh Post Graduate Institute of Biomedical Science and Research (SBSPGI), Dehradun 248161 (UK), India ' State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, Urumqi, 830011 Xinjiang, China; Laboratory of Environmental Pollution and Bioremediation, Xinjiang Institute of Ecology and Geography, Chinese Academy of Science, Urumqi, 830011 Xinjiang, China

Abstract: Current research focused transformation of arsenic, As (III) to As (V) in soil by an efficient bacterial strain (XS9) and successfully transformed 95.4% of As (III) in the soil contaminated with 50 mg/kg and significantly reduced the bioavailable As. After bacterial transformation, we applied two different biochar, produced at 350°C and 650°C at the rate of 2.5, 5 and 10% in the soil, for sorption or stabilization of As (V). We observed that a concentration of 2.5% and 5% biochar efficiently stabilizes the As content as compared to control. Additional, leaching of the treated soil sample was done into the soil column; we found that soil leachate collected at the different time interval (up to 72 h) does not have any soluble/available As. Further, arsenite resistance gene (aioA-gene) was also found in this species (XS9), and strain were identified using 16s rRNA gene sequencing.

Keywords: arsenic; arsenic oxidation; biochar; soil amendment; environments.

DOI: 10.1504/IJEWM.2018.092721

International Journal of Environment and Waste Management, 2018 Vol.21 No.2/3, pp.155 - 171

Available online: 21 Jun 2018 *

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