Title: Filtration of circulating tumour cells MCF-7 in whole blood using non-modified and modified silicon nitride microsieves

Authors: Thien Dien To; An Thu Thi Truong; Anh Tuan Nguyen; Tin Chanh Duc Doan; Chien Mau Dang

Addresses: Laboratory for Nanotechnology, Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam

Abstract: Circulating tumour cells (CTCs) are extremely rare cancer cells in blood stream. The detection of CTCs is an important marker in understanding metastatic risk, disease progression and treatment efficacy. In this study, SiN microsieves were used for detection and capture of MCF-7 cells from whole blood samples based on specific binding of the antibody and size of cells. In the first experiments, the non-modified SiN microsieves were used to capture the MCF-7 cells in phosphate-buffered saline (PBS), whole blood which was incubated with monoclonal anti-cytokeratin - FITC (MACF) antibody, herein the antibody had a specific affinity with MCF-7 cells. MCF-7 cells were captured on the microsieve thanks to their larger size, whereas the smaller blood cells could be discarded. In the second experiments, the SiN microsieves were modified with (3-aminopropyl)triethoxysilane (APTES), glutaraldehyde (GTA) and immobilised with MACF antibody. In this case, MCF-7 cells in PBS, whole blood were filtered directly through the modified SiN microsieves. MCF-7 cells were captured selectively basing on size of cells and the specific antibody binding. After cell filtration, the fluorescent microscopy was used to detect MCF-7 cells on the SiN microsieves in both experiments. This is the first report to demonstrate that the SiN microsieves with surface modification could be used to capture MCF-7 breast cancer cells in human whole blood.

Keywords: silicon nitride microsieves; surface modification; glutaraldehyde; APTES; MCF-7; breast cancer; filtration.

DOI: 10.1504/IJNT.2018.089558

International Journal of Nanotechnology, 2018 Vol.15 No.1/2/3, pp.39 - 52

Available online: 12 Jan 2018 *

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