Title: Phytofabrication, characterisation and antimicrobial studies of gold nanoparticles using Cannabis sativa (Bhang), leaf extract

Authors: Neeraj Sharma; Shailendra Pratap Singh; Uma Rani Sharma

Addresses: Department of Chemistry, GLA University, 17 km stone, NH-2, Mathura Delhi Road, PO: Chauma, Mathura-281406 (UP), India; Department of Mechanical Engineering, GLA University, 17 km stone, NH-2, Mathura Delhi Road, PO: Chauma, Mathura-281406 (UP), India ' Department of Chemistry, GLA University, 17 km stone, NH-2, Mathura Delhi Road, PO: Chauma, Mathura-281406 (UP), India; Department of Mechanical Engineering, GLA University, 17 km stone, NH-2, Mathura Delhi Road, PO: Chauma, Mathura-281406 (UP), India ' Department of Chemistry, GLA University, 17 km stone, NH-2, Mathura Delhi Road, PO: Chauma, Mathura-281406 (UP), India; Department of Mechanical Engineering, GLA University, 17 km stone, NH-2, Mathura Delhi Road, PO: Chauma, Mathura-281406 (UP), India

Abstract: Phytofabrication of gold nanoparticles by plant extract of cannabis sativa is at present under exploitation for the reason to develop environmentally benign nanoparticles synthesis to avoid adverse effects in biomedical applications. Aqueous solution of HAuCl₄ 4H₂O was taken to react with different concentration of ethanolic leaf extract of the plant. A fixed ratio of plant extract and metal ion was mixed which in turn showed the change in colour of the solution leading to the inference that nanoparticles are formed. The characterisation of nanoparticles was done by UV-VIS Spectrophotometer, FTIR, and TEM. The particles synthesised were of the size ranging from 20-100 nm. The solution showed a peak at 560 nm on UV-VIS spectrum corresponding to the surface Plasmon resonance of gold nanoparticles. The shape of the nanoparticles was spherical according to transmission electron microscopy. In these studies, the antimicrobial studies of the AuNPS were exploited against different micro-organisms. The results of UV-VIS, FT-IR and TEM confirm that the leaves extract of Cannabis sativa can be used to produce gold nanoparticles with significant amount of antimicrobial activity.

Keywords: gold nanoparticles; leaf extract; anti-oxidant; cannabis sativa; TEM; FTIR; UV-VIS.

DOI: 10.1504/IJNP.2017.089186

International Journal of Nanoparticles, 2017 Vol.9 No.3, pp.143 - 152

Received: 05 Nov 2016
Accepted: 09 Mar 2017
Published online: 09 Jan 2018 *

Full-text access for editors Full-text access for subscribers Purchase this article Comment on this article