Title: Cellulase production from thermochemically pretreated Chenopodium album

Authors: Anand Prakash; Vinay Sharma; Shivani Sharma; Arindam Kuila

Addresses: Department of Bioscience and Biotechnology, Banasthali University, Rajasthan-304022, India ' Department of Bioscience and Biotechnology, Banasthali University, Rajasthan-304022, India ' Department of Bioscience and Biotechnology, Banasthali University, Rajasthan-304022, India ' Department of Bioscience and Biotechnology, Banasthali University, Rajasthan-304022, India

Abstract: Lignocelllulosic biofuel production is the area of focus of different researchers. Cellulase mediated saccharification of delignified biomass is the rate limiting stage in biofuel generation. For enzymatic hydrolysis cellulase is key enzyme. There are several researches going on for cost effective cellulose production. In the current research, Chenopodium album was subjected to dilute sodium hydroxide treatment on 120°C. Efficiency of pretreatment was evaluated through scanning electron microscopy (SEM), X-ray diffraction (XRD) and biochemical composition analysis. After pretreatment, pretreated biomass was rinsed with tap water and was subsequently kept at 70°C for complete water removal. The biomass after complete water removal was applied for cellulase production using Fusarium oxysporum. Cellulase production was maximised using statistical technique. Within two days, highest cellulase production of 1.51 IU/mL was attained. The above study can be useful for efficient cellulase production for cost effective biofuel production.

Keywords: chenopodium album; thermochemical pretreatment; X-ray diffraction; XRD; scanning electron microscopy; SEM; cellulase production.

DOI: 10.1504/IJRET.2017.088979

International Journal of Renewable Energy Technology, 2017 Vol.8 No.3/4, pp.305 - 314

Available online: 21 Dec 2017 *

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