Title: Bioconversion of Caulerpa racemosa derived galactose rich fraction to D-Tagatose using L-arabinose isomerase from Lactobacillus plantarum MTCC1407

Authors: J. Jayamuthunagai; P. Gautam; M. Chakravarthy; G. Srisowmeya

Addresses: Centre for Biotechnology, Anna University, Chennai, Tamil Nadu – 600025, India ' Centre for Biotechnology, Anna University, Chennai, Tamil Nadu – 600025, India ' Centre for Biotechnology, Anna University, Chennai, Tamil Nadu – 600025, India ' Centre for Biotechnology, Anna University, Chennai, Tamil Nadu – 600025, India

Abstract: A new source of galactose isolated from Caulerpa racemosa is used as raw material for D-tagatose conversion. D-galactose from Caulerpa derived galactose rich fraction (C-GF), which is 99.1% pure is employed in enzymatic bioconversion for D-tagatose using L-arabinose isomerase (l-AI) from Lactobacillus plantarum MTCC1407. The enzyme fraction with l-AI activity is purified to an apparent homogeneity evident on SDS-PAGE with a molecular mass of 59 kDa. Maximum l-AI activity of enzyme was observed at 50°C, pH 7.0 with 20 mM Mg2+ as cofactor. The potential of C-GF to act as D-tagatose feedstock was tested with varying concentrations of C-GF in 50 mM phosphate buffer added with obtained l-AI enzyme. This is compared with the results of pure D-galactose conversion using the same l-AI for better understanding of C-GF as tagatose raw material. Maximum equilibrium of 34.5% tagatose to C-GF galactose was obtained after 40 h producing 0.33 g tagatose/g galactose in C-GF.

Keywords: D-tagatose; L-arabinose isomerase; l-AI; Lactobacillus plantarum; D-galactose; Caulerpa racemosa.

DOI: 10.1504/IJMPT.2017.084956

International Journal of Materials and Product Technology, 2017 Vol.55 No.1/2/3, pp.225 - 235

Available online: 16 Jun 2017

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