Open Access Article

Title: Advances in high-throughput screening technology for toxicology

Authors: Chia-Wen Hsu; Ruili Huang; Matias S. Attene-Ramos; Christopher P. Austin; Anton Simeonov; Menghang Xia

Addresses: National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892-3375, USA ' National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892-3370, USA ' National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892-3375, USA ' National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892-3370, USA ' National Center for Advancing Translational Sciences, National Institutes of Health, 9800 Medical Center Drive, Bethesda, MD 20892-3370, USA ' National Institutes of Health, National Center for Advancing Translational Sciences, 9800 Medical Center Drive, Bethesda, MD 20892-3375, USA

Abstract: The US Tox21 collaboration utilises a quantitative high-throughput screening (qHTS) platform to efficiently profile a large number of environmental chemicals. qHTS combined with informatics facilitates chemical prioritisation for further in-depth toxicity testing and development of computational models to predict chemical toxicity. The NIH Chemical Genomics Center (NCGC), now part of the National Center for Advancing Translational Sciences (NCATS), is a key contributor during all phases of the Tox21 collaboration, from assay development and compound screening to data analysis and model building. Since 2011, the Tox21/NCGC has been profiling the phase 2 Tox21 library of approximately 10,000 (10K) environmental chemicals and drugs. The advances in HTS assays and qHTS screens against the Tox21's and other chemical libraries are described in this review.

Keywords: cytotoxicity; environmental chemicals; drugs; high-throughput screening; nuclear receptors; pharmacology; quantitative HTS; qHTS; structure-activity relationship; SAR; stress-response pathways; Tox21 10K library; Tox21 datasets; chemical toxicology; toxicology testing; chemical libraries.

DOI: 10.1504/IJRAM.2017.082562

International Journal of Risk Assessment and Management, 2017 Vol.20 No.1/2/3, pp.109 - 135

Available online: 27 Feb 2017 *