Title: Viability and topographical analysis of microencapsulated β-cells exposed to a biotransformed tertiary bile acid: an ex vivo study

Authors: Armin Mooranian; Rebecca Negrulj; Hani Al-Salami

Addresses: Biotechnology and Drug Development Research Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Perth, Western Australia, Australia ' Biotechnology and Drug Development Research Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Perth, Western Australia, Australia ' Biotechnology and Drug Development Research Laboratory, School of Pharmacy, Curtin Health Innovation Research Institute, Curtin University, Perth, Western Australia, Australia

Abstract: Microencapsulated pancreatic β-cells have been studied as an ideal system for delivering insulin. However, the clinical applications have not been achieved. We tried multiple excipients/ratios and various methods for producing suitable microcapsules, but success remains limited. A recent study used the tertiary bile acid, taurocholic acid (TCA), to improve the rectal delivery of insulin while another study used TCA to improve the oral delivery of heparin. Thus, in this study, we used TCA in different formulations, using a new microencapsulating method, to study its suitability for β-cell microencapsulation. Using a methodology at set temperature of 37°C, amplitude of 1.5 and a total gelation time of 21 minutes, ten different formulations were examined (with or without TCA) and all formulations showed similar cell viability, which was enhanced by TCA addition. This suggests potential benefits of our microencapsulating parameters and TCA in β-cells microencapsulation.

Keywords: microencapsulation; pancreatic NIT-1 beta cells; taurocholic acid; TCA; inflammation; diabetes; insulin delivery; drug delivery; biotransformation; tertiary bile acid; ex vivo study; microcapsules; rectal delivery; oral delivery; heparin; temperature; gelation time; cell viability.

DOI: 10.1504/IJNBM.2016.079684

International Journal of Nano and Biomaterials, 2016 Vol.6 No.2, pp.74 - 82

Received: 17 Oct 2015
Accepted: 03 Jan 2016
Published online: 09 Oct 2016 *

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