Title: In vitro physicochemical characterisation of doxorubicin-loaded poly (ε-caprolactone)

Authors: R. PrashanthKumar; Jagadeesh G. Hiremath

Addresses: Department of Pharmaceutics, East West College of Pharmacy, Bangalore-560 091, Karnataka, India ' Department of Pharmaceutical Sciences, Ibn Sina National College of Medical Sciences, Jeddah, 21418, P.O. Box 31906, Kingdom of Saudi Arabia

Abstract: In the present study, doxorubicin (DOX)-loaded poly (ε-caprolactone) (PCL) nanoparticles were formulated using the nanoprecipitation technique and their physicochemical properties were evaluated. The size of the nanoparticles was found to be 338-472 nm. The percentage yield obtained was 85.1%-87.8% while the percentage of drug loading was 4%-24%. However, the percentage entrapment efficiency was 81%-88%. The nanoparticles showed characteristic bands at 3439-3451 cm−1 (N-H stretch), 1617-1624 cm−1 (N-H bending 1°amine), 2924-2962 cm−1 (C-H sp3 stretch), and 1287-1295 cm−1 (C-O stretch); this was approximately similar to that of the physical mixture. The only endothermic peak of PCL in the formulation was observed in the range of 59 to 64°C. The characteristic X-ray diffraction peaks of the formulations identified only the polymeric intensive peaks. The different rod shaped nanoparticles were observed under the scanning electron microscope. The results from in vitro drug release studies showed that 20%-28% of the drug was released from the nanoparticles at 24 h at the initial stage; however, approximately 65%-80% of the drug was released at 240 h.

Keywords: doxorubicin; DOX; poly (epsilon-caprolactone); PCL nanoparticles; nanotechnology; physicochemical characterisation; nanoprecipitation; drug loading; percentage entrapment efficiency; in vitro drug release.

DOI: 10.1504/IJNBM.2016.079683

International Journal of Nano and Biomaterials, 2016 Vol.6 No.2, pp.63 - 73

Received: 02 Feb 2015
Accepted: 31 Dec 2015

Published online: 09 Oct 2016 *

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