Title: Immunoassays based on Pt nanoparticles with peroxidase activity

Authors: Jong-Min Park; Ha-Wook Jung; Young Wook Chang; Hyung-Seok Kim; Min-Jung Kang; Jae-Chul Pyun

Addresses: Department of Material Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 120-742, South Korea ' Department of Material Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 120-742, South Korea ' Department of Material Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 120-742, South Korea ' Department of Material Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 120-742, South Korea ' Korea Institute of Science and Technology (KIST), Seoul, 02792, South Korea ' Department of Material Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 120-742, South Korea

Abstract: In this work, Pt nanoparticles were synthesised according to the citrate reduction process. The physical properties of these Pt nanoparticles were characterised using dynamic laser scattering (DLS), zeta-potential analysis, thermal gravimetric analysis (TGA), and TEM. The peroxidase activity of the Pt nanoparticles was also analysed using a chromogenic substrate (TMB) and a luminescent probe (luminol). For application to immunoassays, Pt nanoparticles were coupled with antibodies via electrostatic interaction, and the coupling conditions were optimised at different pH conditions. Finally, the applicability of Pt nanoparticles coupled with anti-hCG (human chorionic gonadotropin) antibodies was demonstrated for the immunoassay of hCG for the medical diagnosis of pregnancy.

Keywords: Pt nanoparticles; platinum nanoparticles; immunoassays; antibodies; coupling; hCG; peroxidase activity; nanotechnology; human chorionic gonadotropin; pregnancy diagnosis.

DOI: 10.1504/IJNT.2016.077090

International Journal of Nanotechnology, 2016 Vol.13 No.4/5/6, pp.402 - 412

Received: 08 May 2021
Accepted: 12 May 2021

Published online: 19 Jun 2016 *

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