Title: Collagen-based scaffold as a delivery system for a niacinamide dominated formulation without loss of resistance against enzymatic degradation
Authors: Quenton Wessels
Addresses: Clinical Anatomy Learning Centre, Lancaster Medical School, Lancaster University, Lancaster, LA1 4YB, UK
Abstract: Exogenous factors aimed at promoting fibroblast activity might hold the key to improving the clinical outcome of chronic wounds. The current study explores the feasibility to use a collagen-based scaffold as a delivery system for a niacinamide dominated formulation in vivo. The combined use of niacinamide, L-carnosine, hesperidin and a HSP70 homologue is known to promote fibroblast activity in vitro. Scaffold mediated wound healing was assessed in 16 female Sprague-Dawley rats that received both a control scaffold and an enhanced scaffold. The test scaffolds presented with a higher fibroblast count (60.49 ± 9.90% of the total cell infiltrate) on day 7 compared that of the control scaffolds (42.62 ± 13.60%) but was found to be statistically insignificant. However, the addition of these active components did not compromise the in vivo resistance against enzymatic degradation nor alter the scaffold microenvironment deleteriously.
Keywords: collagen scaffold; niacinamide; L-carnosine; hesperidin; HSP70 homologue; enzymatic degradation; delivery systems; fibroblast activity; chronic wounds; wound healing.
International Journal of Biomedical Engineering and Technology, 2016 Vol.20 No.4, pp.330 - 343
Received: 16 May 2015
Accepted: 21 Sep 2015
Published online: 17 May 2016 *