Title: Functionalised magnetic beads on gold surface for C-reactive protein detection

Authors: H. Chammem; I. Hafaid; M. Ardhaoui; L. Mora; O. Meilhac; A. Abdelghani

Addresses: Carthage University, Nanotechnology Laboratory, National Institute of Applied Science and Technology, Centre Urbain Nord, Bp676, Charguia Cedex 1080, Tunisia ' Carthage University, Nanotechnology Laboratory, National Institute of Applied Science and Technology, Centre Urbain Nord, Bp676, Charguia Cedex 1080, Tunisia ' Carthage University, Nanotechnology Laboratory, National Institute of Applied Science and Technology, Centre Urbain Nord, Bp676, Charguia Cedex 1080, Tunisia ' Inserm U1148 Laboratory for Vascular Translational Science (LVTS), Institut Galillée, Université Paris 13 Sorbonne Paris Cité, Villetaneuse 93430, France ' Inserm U1188 Diabète athérothrombose Thérapies Réunion Océan Indien (DéTROI), Université de La Réunion, CHU de La Réunion, Saint-Denis, France ' Carthage University, Nanotechnology Laboratory, National Institute of Applied Science and Technology, Centre Urbain Nord, Bp676, Charguia Cedex 1080, Tunisia

Abstract: In this work, we used carboxylic acid groups to functionalise magnetic beads for biomarker detection. A disposable electrochemical biosensor based on functionalised magnetic beads for C-reactive protein detection was developed. Anti-CRP antibody was grafted on magnetic beads after EDC/NHS activation step. The functionalised magnetic beads layer and the molecular structure of the biosensor were then characterised by Fourier Transform Infrared Spectroscopy (FTIR), UV visible spectrophotometry, cyclic voltammetry and impedance spectroscopy. Impedance spectra were modelised with electric model. The developed biosensor was able to detect CRP antigen in the range of 0.1-10 pg/ml.

Keywords: CRP anitgens; FTIR; impedance spectroscopy; functionalised magnetic beads; gold surfaces; C-reactive protein detection; carboxylic acid; biomarker detection; biomarkers; electrochemical biosensors; impedance spectra; modelling; nanotechnology.

DOI: 10.1504/IJNT.2015.068877

International Journal of Nanotechnology, 2015 Vol.12 No.8/9, pp.552 - 561

Available online: 17 Apr 2015 *

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