Authors: Khadijeh Eskandari; Farideh Gouranlou; Mehdi Kamali; Ali Morsali
Addresses: Nanobiotecnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran ' Science and Biotechnology Research Center, Malek Ashtar University of Technology, Tehran, Iran ' Nanobiotecnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran ' Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, Tehran, Iran
Abstract: A feasible and fast method to fabricate glucose biosensor was developed by immobilisation of glucose oxidase (GOx) to nano-sepiolite-graphene nanocomposite/glassy carbon electrode. The direct electrochemistry of glucose oxidase not containing medioter and worked in air saturated condition for similarity of in vivo usage. The nano-sepiolite-graphene nanocomposite shows a high conductive and biocompatible template for enzyme immobilisation. The immobilised GOx on nanocomposite shows a couple of well defined reversible peak with the anodic and cathodic peak potentials of -430 and -460 mV (vs. Ag/AgCl), corresponding to the FAD/FADH2 redox couple, and with a formal potential of -445 mV in 0.1 M phosphate buffer solution at pH = 7. The linear concentration rang of biosensor is 0.14-3.14 µM, and with detection limit of 0.04 µM. Also, the life time of biosensor is more than four months.
Keywords: graphene; clay; nanosepiolite; glucose oxidase biosensors; graphene-clay nanocomposites; electrochemistry; nanotechnology.
International Journal of Biomedical Nanoscience and Nanotechnology, 2014 Vol.3 No.3, pp.278 - 286
Received: 24 Aug 2013
Accepted: 15 Apr 2014
Published online: 07 Nov 2014 *