Title: Biogenic silver nanoparticles synthesised from Zingiber officinale and its antifungal properties

Authors: Malini Soundararajan; Neha Deora; Lynette Lincoln; Purandhi Roopmani; Shikha Gupta; Rajatha Shambu

Addresses: Department of Biochemistry, Center for Post Graduate Studies, Jain University, 18/3, 9th Main Jayanagar, 3rd Block, Bangalore – 560 011, Karnataka, India ' ICMR, Biochemistry Lab, Desert Medicine Research Centre, New Pali Road, Jodhpur – 342 005, Rajasthan, India ' C/o. Mrs. Malini Soundararajan, Department of Biochemistry, Center for Post Graduate Studies, Jain University, 18/3, 9th Main Jayanagar, 3rd Block, Bangalore – 560 011, Karnataka, India ' C/o. Mrs. Malini Soundararajan, Department of Biochemistry, Center for Post Graduate Studies, Jain University, 18/3, 9th Main Jayanagar, 3rd Block, Bangalore – 560 011, Karnataka, India ' C/o. Mr. Bharat Prasad, Ganapati Trading Company, #146A, Sheshapur A, Gorakhpur – 273 005, Uttar Pradesh, India ' C/o. Mrs. Malini Soundararajan, Department of Biochemistry, Center for Post Graduate Studies, Jain University, 18/3, 9th Main Jayanagar, 3rd Block, Bangalore – 560 011, Karnataka, India

Abstract: A silver particle at the nanoscale level behaves as an effective antimicrobial agent and offers numerous applications in biosensing and medicine. The current study unveils the effect of rhizome of Zingiber officinale and its silver nanoparticles against the growth and hydrolytic enzyme of two lethal moulds, Alternaria alternata and Curvularia lunata. The rhizome of Zingiber officinale was extracted under aseptic conditions to get cold distilled water and silver nanoparticle extracts in order to check the effect on inhibition of cell mass formation and protease activity of Curvularia lunata and Alternaria alternata. The formation of silver nanoparticles was confirmed by UV-Vis absorption spectroscopy and scanning electron microscopy. The obtained results showed that the highest tested concentration (2%) of Zingiber officinale in cold distilled water and silver nanoparticle extracts strongly inhibited the cell mass formation as well as protease activity in test organisms. The silver nanoparticle extract showed potent antifungal activity when compared to cold distilled water extract. The study unravels the antifungal property of Zingiber officinale and its biogenically synthesised silver nanoparticles that can be exploited further for therapeutical and other industrial applications.

Keywords: Alternaria alternate; biogenic silver nanoparticles; antifungal properties; Curvularia lunata; nanotechnology; Zingiber officinale; cold distilled water; CDW; silver nitrate; antimicrobial agents; rhizomes; cell mass formation; protease activity.

DOI: 10.1504/IJBNN.2014.065471

International Journal of Biomedical Nanoscience and Nanotechnology, 2014 Vol.3 No.3, pp.251 - 261

Received: 14 Sep 2012
Accepted: 08 Oct 2013
Published online: 07 Nov 2014 *

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