Title: The preparation and characteristic of poly(lactide co-glycolide) microspheres as novel antigen delivery systems

Authors: Hsueh-Ju Lin; Ji-Hung Wang; Chun-Yu Wang; Yi-Chang Wu

Addresses: Institute of Public Health and Department of Public Health, National Yang-Ming Medical University, Taipei, Taiwan; Food and Drug Administration, Department of Health, Executive Yuan, No. 161-2, Kunyang St., Nangang Dist., Taipei City 115, Taiwan ' Department of Cardiology, Buddhist Tzu Chi General Hospital, Hualien, Taiwan ' Department of Pharmacy, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan ' Institute of Aerospace and Diving Medicine, National Defense Medical Center, and Deputy Surgeon General, Bureau of Medical Affairs, Ministry of National Defense, Taiwan

Abstract: Polymeric microspheres with encapsulated antigens or medicine have become well established in the last decade as potent delivery systems. Our novel PLGA microspheres were prepared using a w/o/w solvent evaporation process in the presence of the anionic surfactants, including poly-L-lysine (PLL), cetyltrimethylammonium (CTAB), polyethyleneimine (PEI). High lamellae incorporation efficiency (97%), protein-adsorbed on PLA lamellae capacity (240 μg/mg), efficiency (93%) and encapsulated by PLGA formatted microspheres yield rate (77%) are successfully produced. The PEI modified PLA lamellae (240.2, 20) have more significantly increased proteins loading capacity (μg/mg) and in vitro 50% amount release time (day) than CTAB-modified (83.8, 0.5), PLL-modified (26.0, 0.5), and unmodified lamellae (10.1, 0.3), respectively. Also the sustained release effect over four weeks of FTIR-OVA microspheres was obtained by PEI modified PLA lamellae. In vitro release profiles show more active protein sustain release from PEI modified PLA lamellae than OVA-PLA only microspheres. Antigen binding to the charged PLGA microspheres was influenced by both electrostatic interaction and by other mechanisms, including hydrophobic interactions.

Keywords: entrapped efficiency; microencapsulation; drug delivery systems; PLA; poly(lactic acid); PLGA microspheres; poly(DL-lactide-co-glycolide); PLL; poly-L-lysine; CTAB; cetyltrimethylammonium; PEI; polyethyleneimine; antigen delivery systems; polymeric microspheres; nanotechnology; antigen binding.

DOI: 10.1504/IJNT.2013.058116

International Journal of Nanotechnology, 2013 Vol.10 No.10/11, pp.870 - 890

Available online: 08 Dec 2013 *

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