Title: Biosynthesis of monodispersed silver nanoparticles and their activity against Mycobacterium tuberculosis

Authors: Afreen Banu; Vandana Rathod

Addresses: Department of Microbiology, Gulbarga University, Gulbarga-585106, Karnataka, India ' Department of Microbiology, Gulbarga University, Gulbarga-585106, Karnataka, India

Abstract: In this study, we report the characterisation of biosynthesised silver nanoparticles and investigate the antibacterial effects of biologically synthesised silver nanoparticles (AgNPs) on Mycobacterium tuberculosis. There is an urgent need to develop new anti-tuberculosis drugs, highlighted by the ongoing rise in tuberculosis (TB) cases worldwide. One worrying factor in the current TB problem is the prevalence of multi-drug resistant (MDR) strains. Increased resistance of Mycobacterium tuberculosis (M. tuberculosis) to antibiotics has led the researchers to develop a good antibacterial agent to overcome this problem. A colorimetric, microplate-based Alamar Blue assay (MABA) method was used to determine the MICs of isoniazid (INH), rifampin, streptomycin (SM), and ethambutol (EMB) for M. tuberculosis clinical isolates and silver nanoparticles against multi-drug resistance strains of M. tuberculosis isolated from MABA method.

Keywords: Rhizopus stolonifer; AgNPs; Mycobacterium tuberculosis; TB; biosynthesis; silver nanoparticles; nanotechnologys; antibacterial agents; anti-tuberculosis drugs; drug resistance; isoniazid; rifampin; streptomycin; ethambutol.

DOI: 10.1504/IJBNN.2013.054507

International Journal of Biomedical Nanoscience and Nanotechnology, 2013 Vol.3 No.1/2, pp.211 - 220

Published online: 12 Jul 2014 *