Title: Spot matching in 2D electrophoresis experiments

Authors: Carlos Fernandez-Lozano; Alvaro Rodriguez-Tajes; Cristina Ruiz-Romero; Jose A. Seoane

Addresses: Information and Communications Technologies Department, School of Computer Science, University of A Coruña, Campus de Elviña, S/N 15071 A Coruña, Spain ' Information and Communications Technologies Department, School of Computer Science, University of A Coruña, Campus de Elviña, S/N 15071 A Coruña, Spain ' Complejo Hospitalario Universitario de A Coruña (CHUAC), As Xubias, num 84, 15006 A Coruña, Spain ' Information and Communications Technologies Department, School of Computer Science, University of A Coruña, Campus de Elviña, S/N 15071 A Coruña, Spain

Abstract: Several computational approaches try to help the researchers to establish the correspondence between pairs of proteins of multiple 2D gel images. In this work, an approach based on block-matching techniques is developed. Its main characteristic is that it does not need to perform the whole alignment between two images considering each protein separately. It can be concluded that this method works over broad range of proteomic images, although they have a high level of difficulty. After matching the proteins between 2D gel images, protein expression profiles must be done and data mining techniques must be applied for extracting non-trivial information from the data obtained. This way, this method can improve the flow of 2D gel analysis in aid of the association studies and the diagnosis. In the future, this information obtained after electrophoresis process can lead to an accurate diagnosis.

Keywords: 2-D gel electrophoresis; block matching; proteomics; differential expression analysis; computer-assisted image processing; image segmentation; association studies; data analysis; data mining; spot matching; image registration; protein pairs; proteins; multiple gel images; biomedicine.

DOI: 10.1504/IJDATS.2013.053681

International Journal of Data Analysis Techniques and Strategies, 2013 Vol.5 No.2, pp.198 - 213

Available online: 04 May 2013 *

Full-text access for editors Access for subscribers Purchase this article Comment on this article