Title: Glucose biosensor based on platinum nanowires: a clinical study

Authors: Thi Hong Thuy Phan; Phu Duy Tran; Xuan Thanh Tung Pham; Ngoc Thuy Duong Dang; Viet Long Nguyen; Van Man Tran; Truong Giang Bui; Mau Chien Dang; Duy Hien Tong

Addresses: Laboratory for Nanotechnology, Vietnam National University, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' University of Science Ho Chi Minh, Vietnam National University, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam ' Laboratory for Nanotechnology, Vietnam National University, Community 6, Linh Trung Ward, Thu Duc District, Ho Chi Minh City, Vietnam

Abstract: We describe the process of modification of the platinum (Pt) nanowire surface and subsequent immobilisation of enzyme glucose oxidase (GOx) on Pt nanowires (PtNWs) for direct glucose measurement. A cyclic voltammetry technique was used to measure electrochemical oxidation between GOx and glucose. PtNW arrays were first fabricated and then functionalised with cysteamine, which in turn acted as a chemical linkage for the enzyme conjugation. Afterward, the enzyme modified PtNWs arrays were subjected to the model test to check the effect of the process modification. The cyclic voltammograms of GOx immobilised Pt chips in PBS (1M, pH of 7) showed the most stable result. The enzymatic modified PtNW electrodes were then successfully determined glucose in diabetic blood samples with the concentrations from 125 µM to 16.5 mM, a deviation of ± 3%. The obtained results clearly indicate the potential of a next-generation of glucose sensor based on PtNW in application diabetic diagnosing.

Keywords: platinum nanowires; nano-biosensors; glucose biosensors; diabetic blood; clinic study; nanotechnology; glucose measurement; diabetes diagnosis.

DOI: 10.1504/IJNT.2013.053129

International Journal of Nanotechnology, 2013 Vol.10 No.3/4, pp.166 - 177

Available online: 05 Apr 2013 *

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