Title: Injection techniques for bulk cell seeding decellularised vascular scaffolds

Authors: William S. Sheridan; Garry P. Duffy; Bruce P. Murphy

Addresses: Department of Mechanical and Manufacturing Engineering, Trinity Centre for Bioengineering, Parsons Building, Trinity College Dublin, College Green, Dublin 2, Ireland. ' Department of Anatomy, Royal College of Surgeons in Ireland, 123 St Stephens Green, Dublin 2, Ireland. ' Department of Mechanical and Manufacturing Engineering, Trinity Centre for Bioengineering, School of Engineering, Parsons Building, Trinity College Dublin, College Green, Dublin 2, Ireland

Abstract: Tissue engineering small diameter vascular grafts requires excessive culture times to produce an appropriate extracellular matrix (ECM). The use of decellularised exogenous tissue as a scaffold has the potential to overcome this limitation due to the preformed ideal ECM architecture that remains after decellularisation. While this scaffold reduces ECM maturation times cell seeding is difficult due to the high density of decellularised tissue, particularly within the medial layer of the scaffold. In this study, we investigated the possibility of direct injection of vascular cells within decellularised porcine carotid arteries through the use of micro-needles inserted into the medial layer prior to decellularisation. The results indicate that these injection methods are feasible and provide a method for quickly bulk cell seeding decellularised scaffolds.

Keywords: tissue engineering; decellularisation; extracellular matrix; cell seeding; smooth muscle cells; bulk seeding; direct injection; carotid arteries; micro-needles; vascular graft; biomaterials; vascular scaffolds; vascular cells.

DOI: 10.1504/IJNBM.2012.050310

International Journal of Nano and Biomaterials, 2012 Vol.4 No.2, pp.96 - 107

Received: 03 Feb 2012
Accepted: 21 Aug 2012

Published online: 14 Nov 2012 *

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