Title: Adhesion and survival of electrogenic cells on gold nanopillar array electrodes

Authors: Dorothea Brüggemann; Kristin E. Michael; Bernhard Wolfrum; Andreas Offenhäusser

Addresses: PGI-8/ICS-8, Forschungszentrum Jülich GmbH, Jülich, Germany; JARA – Fundamentals of Future Information Technology, 52425 Jülich, Germany; CRANN – The Naughton Institute, School of Physics, Trinity College Dublin, Dublin 2, Ireland. ' Deceased; formerly of: PGI-8/ICS-8, Forschungszentrum Jülich GmbH, Jülich, Germany; JARA – Fundamentals of Future Information Technology, 52425 Jülich, Germany. ' PGI-8/ICS-8, Forschungszentrum Jülich GmbH, Jülich, Germany; JARA – Fundamentals of Future Information Technology, 52425 Jülich, Germany. ' PGI-8/ICS-8, Forschungszentrum Jülich GmbH, Jülich, Germany; JARA – Fundamentals of Future Information Technology, 52425 Jülich, Germany

Abstract: Cell-electrode interfaces play a critical role in extracellular recording. Enlarging the electrode surface area with nanostructures yields higher signal-to-noise-ratios due to lower interface impedance. Adhesion and viability of various cell types on large-scale gold nanopillar electrodes to improve cell-electrode coupling were investigated. Cardiac muscle and human embryonic kidney cells survived and adhered well on gold nanopillars. The muscle cells even protruded into inter-pillar cavities with diameters below 100 nm. However, an unexpectedly low viability and adhesion of primary rat neurons was observed on nanopillars. A cross-sectional analysis of the cell-nanopillar interface showed large distances between neuronal cell bodies and nanopillars, whereas the neurites adhered tightly. Furthermore, actin assembly within the neuronal growth cones was modified on nanopillars. In summary, the adhesion response of the investigated cell lines will be beneficial for improved extracellular signalling, whereas a better understanding of neuronal responses to nanotopographies is required to enhance the neuronal viability.

Keywords: biosensors; nanostructured electrodes; anodic alumina templates; gold nanopillars; cell adhesion; cell viability; neuronal cells; neuronal networks; nanotopography; cell-electrode interface; biomaterials; electrogenic cells; extracellular signalling; nanotechnology; nanomaterials; cardiac muscle cells; embryonic kidney cells.

DOI: 10.1504/IJNBM.2012.050304

International Journal of Nano and Biomaterials, 2012 Vol.4 No.2, pp.108 - 127

Available online: 14 Nov 2012 *

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