Title: Application of RAPD markers in fish: Part I – some genera (Tilapia, Sarotherodon and Oreochromis) and species (Oreochromis aureus and Oreochromis niloticus) of Tilapia

Authors: Mohamed M.M. Ahmed, Bahy Ahmed Ali, Samy Y. EI-Zaeem

Addresses: Nucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), Mubarak City for Scientific Research & Technology Applications, Alexandria, Egypt. ' Cell Biology and Medical Genetics Department, Research Center for Reproductive Medicine, Shantou University, Medical College, 22 Xin Lin Road, Shantou, Guangdong, 515031, PR China. ' Animal and Fish Production Department, Saba-Bacha, Alexandria University, Alexandria, Egypt

Abstract: Random amplified polymorphic DNA (RAPD) analysis was applied to the four-tilapia species (three genera): redbelly tilapia (Tilapia zillii), white tilapia (Sarotherodon galilaeus), blue tilapia (Oreochromis aureus) and Nile tilapia (Oreochromis niloticus). Twenty random primers – fifteen (10-mer) and five (20-mer) – were used to assay polymorphisms among three genera and between two species. Different RAPD fragment patterns were observed for different genera species. Results showed that there are great differences among the three genera of tilapia fish. Also, data demonstrated that RAPD markers are useful for the systematic investigation at the level of tilapia species.

Keywords: tilapia fish; genera; species; RAPD; analysis.

DOI: 10.1504/IJBT.2004.004615

International Journal of Biotechnology, 2004 Vol.6 No.1, pp.86 - 93

Published online: 26 May 2004 *

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