Title: Advanced microscopy techniques for biological imaging

Authors: Serge Monneret; Sophie Brasselet

Addresses: Aix-Marseille Université, CNRS, Institut Fresnel, Campus de Saint-Jérôme, 13013 Marseille, France. ' Aix-Marseille Université, CNRS, Institut Fresnel, Campus de Saint-Jérôme, 13013 Marseille, France

Abstract: We show that using phase and polarisation information in optical microscopy can provide new insights into biomolecular processes for biological functional imaging. Quantitative phase imaging is demonstrated in living cells with promising capabilities in terms of cells composition and morphology studies. Polarisation-resolved non-linear optical microscopy is described in tissues and lipid membranes with emphasis on the specific properties of two-photon excited fluorescence, Second Harmonic Generation and Coherent Anti-Stokes Raman Imaging. These techniques, based on far field microscopy, provide new information at the molecular scale, complementary to more traditional fluorescence microscopy techniques.

Keywords: quantitative phase microscopy; wavefront sensing; nonlinear microscopy; polarisation; biological imaging; biomolecular processes; nanotechnology; tissues; lipid membranes.

DOI: 10.1504/IJNT.2012.045339

International Journal of Nanotechnology, 2012 Vol.9 No.3/4/5/6/7, pp.548 - 566

Received: 08 May 2021
Accepted: 12 May 2021

Published online: 03 Feb 2012 *

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