Title: Growth and behaviour of bovine articular chondrocytes on nanoengineered surfaces: Part I

Authors: Jameel Shaik, Javeed Shaikh Mohammed, Michael J. McShane, David K. Mills

Addresses: Institute for Micromanufacturing, Louisiana Tech University, USA; Biomedical Engineering Department, Louisiana Tech University, USA; Biomedical Engineering Department, School of Bio Sciences & Technology, VIT University, Vellore 632014, Tamil Nadu, India. ' Institute for Micromanufacturing, Louisiana Tech University, USA; Department of Biomedical Technology, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh 11433, Kingdom of Saudi Arabia. ' Institute for Micromanufacturing, Louisiana Tech University, USA; Biomedical Engineering Department, Louisiana Tech University, USA; Department of Biomedical Engineering, Texas A&M University, 335K Zachry Engineering Center, College Station, TX 77843-3120, USA. ' Biological Sciences/Institute for Micromanufacturing, Louisiana Tech University, 1 Arizona, POB 3179, Carson Taylor Hall, Room 141, Ruston, LA 71272, USA

Abstract: Surface modification, using biomaterials to mimic in vivo cell microenvironment, plays an important role in tissue engineering. Current work studies the growth and behaviour of primary bovine articular chondrocytes on layer-by-layer self-assembled nanofilms of 11 different biomaterials, including polyelectrolytes and proteins [poly(styrene sulphonate) (PSS), fibronectin, poly-L-lysine (PLL), poly-D-lysine (PDL), laminin, bovine serum albumin (BSA), chondroitin sulphate (CS), poly(ethyleneimine) (PEI), poly(dimethyldiallylammonium chloride) (PDDA), collagen and poly(ethylene glycol) amine (PEG-NH2)]. Mono-, bi-, and tri-layer nanofilm architectures were deposited on 24-, 96-well plate polystyrene surfaces. Surface roughness of nanofilms was determined using atomic force microscopy. Chondrocytes cultured on nanofilms were analysed using microscopy, live-dead viability assay, and MTT proliferation assay. Statistical analyses of chondrocyte viability and metabolic activity results on mono-, bi-, and tri-layer nanofilm architectures indicate the significant influence of cell seeding density and number of nanofilm layers on the viability and metabolic activity of chondrocytes.

Keywords: polyelectrolytes; proteins; layer-by-layer; self-assembly; multilayers; nanofilms; nanoscale; surface modification; primary bovine articular chondrocytes; nanofilm architectures; surface roughness; atomic force microscopy; cell seeding density; cell viability; cell metabolic activity; nanotechnology; nanoengineered surfaces; biomaterials; tissue engineering.

DOI: 10.1504/IJNT.2011.041438

International Journal of Nanotechnology, 2011 Vol.8 No.8/9, pp.679 - 699

Published online: 22 Jul 2011 *

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