Title: Amelioration of Mn toxicity by Brassinosteroids in Brassica juncea L.

Authors: Renu Bhardwaj, Priyanka Sharma, Nitika Arora, Hardesh Kumar Arora

Addresses: Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India. ' Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India. ' Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India. ' Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar 143005, Punjab, India

Abstract: The effects of 28-homobrassinolide (28-homoBL) on protein content and antioxidative enzyme activities in 7-days old Brassica juncea L. seedlings were studied. The seeds pre-treated with different concentrations of 28-homoBL were germinated in Petriplates supplied with various concentrations of heavy metal (Mn). The activities of superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase and glutathione reductase of 7-days old seedlings were analysed. It was observed that 28-homoBL reduced the toxicity of heavy metal on seedling growth by influencing the protein content and antioxidative enzyme activities. Pre-treatment of 28-homoBL was found to be significantly effective in increasing the activities of all antioxidative enzymes.

Keywords: 28-homobrassinolide; antioxidative enzymes; heavy metal stress; manganese; Indian mustard; Mn toxicity; Brassica juncea L; environmental pollution; protein content; seedlings; seedling growth.

DOI: 10.1504/IJEWM.2011.040969

International Journal of Environment and Waste Management, 2011 Vol.8 No.1/2, pp.114 - 122

Published online: 27 Jun 2011 *

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