Title: Engineering generic vaccine vectors

Authors: Poda Sudhakar, M. Usha Kiranmayi, Selvaraj Sankarpandian, Manyam Srinivasa Rao, M. Vijayalakshmi, K.R.S. Sambasiva Rao

Addresses: Centre for Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, Andhra Pradesh, India. ' Department of Microbiology, Sri Krishnadevaraya University, Ananthapur 515003, Andhra Pradesh, India. ' Centre for Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, Andhra Pradesh, India. ' Centre for Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, Andhra Pradesh, India. ' Department of Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, Andhra Pradesh, India. ' Centre for Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur 522510, Andhra Pradesh, India

Abstract: DNA vaccine engineering has become the favoured strategy for inducing immunity. The molecular precision offered to direct and amplify the immunity by the gene-based vaccines has always been attractive. The design of the vector is the most important aspect to be considered, as several factors such as promoter, enhancer, codon choice and genetic adjuvants are the key elements that control the expression of the cloned antigen gene. The review helps to consider the manufacturing ease and factors that are to be taken into account while constructing the vector.

Keywords: engineering vaccines; promoters; vaccine vectors; DNA vaccine engineering; immunity; enhancers; codon choice; genetic adjuvants; cloned antigen genes; DNA vaccines.

DOI: 10.1504/IJBET.2011.040455

International Journal of Biomedical Engineering and Technology, 2011 Vol.6 No.1, pp.93 - 108

Published online: 21 Jan 2015 *

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