Title: Immobilising of anti-HPV18 and E. coli O157:H7 antibodies on magnetic silica-coated Fe₃O₄ for early diagnosis of cervical cancer and diarrhoea

Authors: Tran Hoang Hai, Le Hong Phuc, Le Khanh Vinh, Bui Duc Long, Truong Thuy Kieu, Nguyen Ngoc Bich, Tran Nguyen Lan, Nguyen Quan Hien, Le Hoang Anh Khoa, Nguyen Ngoc Van Tam

Addresses: Department of Novel and Nanostructure Materials, Ho Chi Minh City Institute of Physic, 01 Mac Dinh Chi Str., Distr. 1, Ho Chi Minh City, Vietnam. ' Department of Novel and Nanostructure Materials, Ho Chi Minh City Institute of Physic, 01 Mac Dinh Chi Str., Distr. 1, Ho Chi Minh City, Vietnam. ' Department of Novel and Nanostructure Materials, Ho Chi Minh City Institute of Physic, 01 Mac Dinh Chi Str., Distr. 1, Ho Chi Minh City, Vietnam. ' Department of Novel and Nanostructure Materials, Ho Chi Minh City Institute of Physic, 01 Mac Dinh Chi Str., Distr. 1, Ho Chi Minh City, Vietnam. ' Department of Novel and Nanostructure Materials, Ho Chi Minh City Institute of Physic, 01 Mac Dinh Chi Str., Distr. 1, Ho Chi Minh City, Vietnam. ' Department of Novel and Nanostructure Materials, Ho Chi Minh City Institute of Physic, 01 Mac Dinh Chi Str., Distr. 1, Ho Chi Minh City, Vietnam. ' Department of Novel and Nanostructure Materials, Ho Chi Minh City Institute of Physic, 01 Mac Dinh Chi Str., Distr. 1, Ho Chi Minh City, Vietnam. ' Department of Chemistry, Ho Chi Minh City University of Pedagogy, 280 An Duong Vuong, 4th Ward, Ho Chi Minh City, Vietnam. ' Soc Son High School, Hon Dat Distr., Kien Giang Province, Vietnam. ' Nguyen Thi Minh Khai High School, 66 B Nguyen Thi Minh Khai, Soc Trang Province, Vietnam

Abstract: This paper presents the synthesis and properties of magnetic silica-coated Fe₃O₄ nanoparticles. Uncoated Fe₃O₄ nanoparticles with an average diameter of 9-16 nm and saturation magnetisation of around 66 emu/g were first prepared by co-precipitation method. After being coated by SiO₂ using the sol-gel method, the diameters of the coated particles ranged from 29 nm to 230 nm and their corresponding saturation magnetisation was reduced to 29 emu/g. The magnetic coated nanoparticles were attached to the monoclonal antibodies of HPV18 and E. coli O157:H7. The TG/DTA analyses indicated that antibodies were attached to the magnetic nanoparticles. The obtained results revealed that magnetic SiO₂-coated Fe₃O₄ nanoparticles can be a promising candidate for the diagnosis of cervical cancer at an early stage with high accuracy.

Keywords: magnetic nanoparticles; diagnostics; uterocervical cancer; E. coli; monoclonal antibodies; escherichia coli; cervical cancer; diarrhoea; cancer diagnosis; sol-gel; nanotechnology.

DOI: 10.1504/IJNT.2011.038214

International Journal of Nanotechnology, 2011 Vol.8 No.3/4/5, pp.383 - 398

Published online: 21 Jan 2011 *

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