Title: Studies on cloning and economic production of recombinant Streptokinase in a novel osmotically inducible E. Coli GJ1158

Authors: K.K. Pulicherla, A. Krishna Satya, V.P.B. Rekha, G.S. Gadupudi, J.B. Peravali, K.R.S. Sambasiva Rao

Addresses: Department of Biotechnology, R.V.R. and J.C. College of Engineering, Chowdavaram, Guntur 522019, Andhra Pradesh, India. ' Centre for Biotechnology, Acharya Nagarjuna University, Guntur 522510, Andhra Pradesh, India. ' Department of Biotechnology, R.V.R. and J.C. College of Engineering, Chowdavaram, Guntur 522019, Andhra Pradesh, India. ' Department of Biology (Microbiology and Molecular Cell Sciences), University of Memphis, Tennessee, USA. ' Department of Biotechnology, Bapatla Engineering College, Bapatla 522001, Andhra Pradesh, India. ' Center for Biotechnology, Acharya Nagarjuna University, Guntur 522510, Andhra Pradesh, India

Abstract: Streptokinase, a 47KDa protein is efficaciously used as a thrombolytic drug. Though it is relatively inexpensive, being a bacterial protein, Streptokinase is antigenic to humans. The low yields obtained in Streptokinase production, and the pathogenic nature of its natural host is the principal reasons to search for a recombinant producer. The report includes successful isolation of the Streptokinase gene from the wild host Streptococcus species MTCC389 and transforming it to E. coli expression hosts BL21(DE3) and GJ1158 through pRSET-A plasmid cloning vector, optimisation of media and analysis of successful expression of recombinant streptokinase in new host.

Keywords: thrombolytic agents; recombinant Streptokinase; Streptococcus species; Fibrinolytic agents; E. coli GJ1158; cloning; economic production; thrombolytic drugs; Streptokinase gene isolation.

DOI: 10.1504/IJBET.2010.034276

International Journal of Biomedical Engineering and Technology, 2010 Vol.4 No.1, pp.29 - 39

Published online: 31 Jul 2010 *

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