Title: A comparison of microbial contact bioassay with conventional elutriate assays for evaluation of wastes hazard

Authors: Svetlana Selivanovskaya, Polina Galitskaya, Sylvia Schnell, Yung-Tse Hung

Addresses: Department of Applied Ecology, Kazan State University, Kremlyevskaya str., 18, Kazan 420008, Russia. ' Department of Applied Ecology, Kazan State University, Kremlyevskaya str., 18, Kazan 420008, Russia. ' Research Center for Biosystems, Land Use and Nutrition, Justus-Liebig University, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany. ' Department of Civil Engineering, University of Cleveland, 1983 E24 St., Cleveland, Ohio 44115-2440, USA

Abstract: The aim of this investigation was to develop a battery of bioassays – contact and elutriate tests with Bacillus pumilus, elutriate tests with Daphnia magna and Paramecium caudatum, for the assessment of the wastes| toxicity. The contact test with B. pumilus was more sensitive than the elutriate test. The estimated LID10 were much higher for Daphnia and Bacillus tests than for Paramecium test. No correlation was determined between the data files of bioassays and of the chemical analysis of the waste. The procedure for classifying the wastes into the hazard classes by calculating LID10 in the Bacillus test was proposed.

Keywords: industrial wastes; Bacillus pumilus; Daphnia magna; Paramecium caudatum; microbial contact bioassay; elutriate assays; hazard evaluation; waste hazards; waste management; waste toxicity.

DOI: 10.1504/IJEWM.2010.033992

International Journal of Environment and Waste Management, 2010 Vol.6 No.1/2, pp.183 - 196

Published online: 06 Jul 2010 *

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