Title: Alkaline lipase production from Enterobacter aerogenes by solid-state fermentation of agro-industrial wastes

Authors: Sanghamitra Mitra, S.K. Khare, Rajni Singh

Addresses: Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi. ' Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi. ' Amity Institute of Microbial Biotechnology, Amity University, Sector-125, Noida, Uttar Pradesh, India

Abstract: Lipases that are stable at alkaline pH are highly desirable for detergent formulations. In the present study, an alkaline lipase was produced from solid-state fermentation of agro-industrial wastes by lipase producer strain of Enterobacter aerogenes. Use of such wastes as substrates instead of commercial synthetic media was done to develop a cost-effective method for lipase production. Fermentation conditions were optimised for maximising enzyme production. The lipase was found to be stable at higher pH 9-11 and temperature 45-60°C ranges. Its incorporation in detergent formulation enhanced the washing efficiency.

Keywords: alkaline lipase; detergent applications; Enterobacter aerogenes; solid-state fermentation; soybean oil cake; agro-industrial waste.

DOI: 10.1504/IJEWM.2010.032017

International Journal of Environment and Waste Management, 2010 Vol.5 No.3/4, pp.410 - 418

Published online: 04 Mar 2010 *

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