Authors: Christopher M. Frenz, Philippe P. Lefebvre
Addresses: Department of Otolaryngology, University of Liege, CHU de Liege, 4000 Liege, Belgium. ' Department of Otolaryngology, University of Liege, CHU de Liege, 4000 Liege, Belgium
Abstract: The POU4F3 transcription factor is expressed in the cochlear and vestibular hair cells of the inner ear and its targeted deletion results in a loss of inner ear hair cells. The DFNA15 truncation mutation has been demonstrated to result in a loss of transcriptional activity, but an increase in the stability of the protein. Molecular modelling is utilised to propose a mechanism of stability enhancement, via an interaction between the truncated POUHD domain and the POUS domain of the transcription factor.
Keywords: molecular modelling; protein stability; deafness; POU4F3 transcription factor; cochlear hair cells; vestibular hair cells; inner ear; DFNA15 truncation mutation; progressive hearing loss.
International Journal of Computational Biology and Drug Design, 2008 Vol.1 No.3, pp.295 - 301
Available online: 26 Nov 2008 *Full-text access for editors Access for subscribers Purchase this article Comment on this article