Title: Growth kinetics of coliform bacteria isolated from food

Authors: Gizem Özlük Çilak; Hayriye Bahar; Ümmü Büşra Yavuz; Eda Nilsun Emin; Sinan Bostan; Hilal Selamoğlu Ata; Kadir Halkman

Addresses: Department of Food Engineering, Faculty of Engineering, Hitit University, Çevre Yolu, Kuzey Kampüs, 19030, Çorum, Turkey ' Food Engineering Department, Ankara University, Turkey ' Food Engineering Department, Ankara University, Turkey ' Food Engineering Department, Ankara University, Turkey ' Food Engineering Department, Ankara University, Turkey ' Maysa Gıda, Istanbul, Turkey ' Food Engineering Department, Ankara University, Turkey

Abstract: It was aimed to obtain the growth kinetic parameters of food related coliform bacteria so that the industry can take action against not only to well-known Escherichia coli but also all. Isolates of Citrobacter freundii, Enterobacter aerogenes, Enterobacter cloacae, Escherichia coli and Klebsiella pneumoniae were obtained from food samples and pure cultures were inoculated to three different media (tryptic soy broth, lauryl sulphate tryptose broth and lactose broth) and incubated at two different temperatures (28°C and 37°C) for six hours. The kinetic factors for exponential phase of each bacterium in all media, at all temperature were determined. This study showed that coliform bacteria acts differently and have different preference of medium and temperature which may end up in masking by competitive flora, thus it is recommended to use at least two different media at two different temperatures in coliform analysis.

Keywords: coliform; Escherichia coli; Klebsiella pneumoniae; Citrobacter freundii; Enterobacter aerogenes; Enterobacter cloacae; kinetic factors; specific growth rate; generation time.

DOI: 10.1504/IJFSNPH.2025.149640

International Journal of Food Safety, Nutrition and Public Health, 2025 Vol.6 No.4, pp.196 - 202

Received: 30 Dec 2019
Accepted: 22 Mar 2021
Published online: 10 Nov 2025 *

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