Title: Bactericidal activity of clove basil essential oil microemulsion on Aeromonas hydrophila

Authors: Phuong Hong Lam; Huyen Da Nguyen Vo; Mai Thi Le; Linh My Thi Truong; Chien Mau Dang; Dung My Thi Dang

Addresses: Institute for Nanotechnology, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam; University of Science, 227 Nguyen Van Cu Street, Ward 4, District 5, Ho Chi Minh City, 700000, Vietnam; Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam ' Institute for Nanotechnology, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam; Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam ' Institute for Nanotechnology, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam; Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam ' Institute for Nanotechnology, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam; Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam ' Institute for Nanotechnology, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam; Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam ' Institute for Nanotechnology, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam; Vietnam National University Ho Chi Minh City, Community 6, Linh Trung Ward, Thu Duc City, Ho Chi Minh City, 700000, Vietnam

Abstract: In this experiment, clove basil essential oil was prepared in microemulsion and its bactericidal activity was evaluated on Aeromonas hydrophila, a common bacterial species that causes hemorrhagic disease in catfish. The results demonstrated that the prepared microemulsion was an oil-in-water type, characterised by transparency, a particle size of 40.61 nm with a low PDI of 0.23. Furthermore, the clove basil essential oil microemulsion had bactericidal activity on A. hydrophila with a minimum inhibitory concentration (MIC) of 1.25 % (v/v). Notably, the microemulsion form of clove basil essential oil displayed improved antibacterial properties compared to its original counterpart. This showed the potential application of clove basil essential oil microemulsion to prevent bacterial diseases in catfish, especially the disease caused by A. hydrophila.

Keywords: clove basil; microemulsion; bactericidal activity; Aeromonas hydrophila; MIC; minimum inhibitory concentration.

DOI: 10.1504/IJNT.2024.146459

International Journal of Nanotechnology, 2024 Vol.21 No.7/8/9/10/11/12, pp.595 - 608

Published online: 30 May 2025 *

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