Article: Effective delignification of cabbage stem by organosolvent pre-treatment and subsequent enzymatic hydrolysis of pre-treated substrate Journal: International Journal of Environment and Waste Management (IJEWM) 2025 Vol.36 No.1 pp.65 - 82 Abstract: Non-edible portions of brassica vegetables are discarded in huge quantities, which is a great concern for causing environmental pollution. In the present study, cabbage stem was utilised for fermentable sugars production through enzymatic hydrolysis. Proximate composition of cabbage stem showed presence of lignocellulosic components (cellulose, hemicellulose and lignin) in appreciable amounts. Different pre-treatment approaches (acid, alkali and organosolvent) have been investigated for the effective delignification of cabbage stem and subsequent enzymatic hydrolysis of pre-treated substrate for better release of fermentable sugars. Organosolvent pre-treatment released highest quantity of cellulose content (69.28 ± 0.58a%) in comparison to acid and alkali pre-treatment. Optimisation of different process conditions for enzymatic saccharification of pre-treated cabbage stem with cellulase enzyme was carried out and maximum yield of reducing sugar content of 782 ± 1.14b mg/g was achieved at 1.5% (w/v) substrate concentration, 50°C temperature, pH 4, usage of 15 U/g enzyme concentration, and incubation time of 240 minutes. Valorisation of cabbage stem as the novel lignocellulosic waste sources into bio-products through enzymatic saccharification process for the mitigation of environmental pollution caused by the accumulation of these household or domestic organic residues has an added advantage. Inderscience Publishers - linking academia, business and industry through research

Title: Effective delignification of cabbage stem by organosolvent pre-treatment and subsequent enzymatic hydrolysis of pre-treated substrate

Authors: Sayari Majumdar; Swagata Ghosh; Jayati Bhowal

Addresses: School of Community Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India ' School of Community Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India ' School of Community Science and Technology, Indian Institute of Engineering Science and Technology, Shibpur, India

Abstract: Non-edible portions of brassica vegetables are discarded in huge quantities, which is a great concern for causing environmental pollution. In the present study, cabbage stem was utilised for fermentable sugars production through enzymatic hydrolysis. Proximate composition of cabbage stem showed presence of lignocellulosic components (cellulose, hemicellulose and lignin) in appreciable amounts. Different pre-treatment approaches (acid, alkali and organosolvent) have been investigated for the effective delignification of cabbage stem and subsequent enzymatic hydrolysis of pre-treated substrate for better release of fermentable sugars. Organosolvent pre-treatment released highest quantity of cellulose content (69.28 ± 0.58^a%) in comparison to acid and alkali pre-treatment. Optimisation of different process conditions for enzymatic saccharification of pre-treated cabbage stem with cellulase enzyme was carried out and maximum yield of reducing sugar content of 782 ± 1.14^b mg/g was achieved at 1.5% (w/v) substrate concentration, 50°C temperature, pH 4, usage of 15 U/g enzyme concentration, and incubation time of 240 minutes. Valorisation of cabbage stem as the novel lignocellulosic waste sources into bio-products through enzymatic saccharification process for the mitigation of environmental pollution caused by the accumulation of these household or domestic organic residues has an added advantage.

Keywords: cabbage stem; cellulase; organosolvent pre-treatment; reducing sugars.

DOI: 10.1504/IJEWM.2025.143561

International Journal of Environment and Waste Management, 2025 Vol.36 No.1, pp.65 - 82

Accepted: 28 Jan 2022
Published online: 30 Dec 2024 *

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Title: Effective delignification of cabbage stem by organosolvent pre-treatment and subsequent enzymatic hydrolysis of pre-treated substrate

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