Title: Simulate the agarose gel of the receptor binding domain in Omicron's spike protein inserted into the pGEM®-T Easy vector using SpeI and SacI restriction enzymes

Authors: Ali Adel Dawood

Addresses: Department of Anatomy, College of Medicine, University of Mosul, Mosul, 00964, Iraq

Abstract: The Omicron variant of SARS-CoV-2 spreads swiftly among humans because of the mutations it carries. Aim: The goal of this study was to find a full design of the polymerase chain reaction (PCR) protocol for determining SARS-CoV-2 using In silico tools. The receptor-binding domain (RBD) of the spike protein is a crucial component. Materials and Methods: RBD interacts with ACE2 receptors on the surface of respiratory lining cells. The linear RBD was inserted into the supercoiled pGEM®-T Easy vector using SpeI and SacI restriction enzymes. The RBD was amplified to perform the conventional PCR technique after designing specific primers. Results: The (pGEM®-T Easy-RBD Mut.) clone was created by inserting the Omicron RBD gene into a supercoiled pGEM®-T Easy commercial vector. The results were predicted using gel electrophoresis. Even though the classic PCR method takes time due to the electrophoresis procedure, it is a very efficient tool for detecting novel strains.

Keywords: Omicron; SARS-CoV-2; vector; RBD; receptor-binding domain; spike.

DOI: 10.1504/IJCBDD.2023.134614

International Journal of Computational Biology and Drug Design, 2023 Vol.15 No.6, pp.518 - 526

Received: 10 Aug 2022
Accepted: 03 Jul 2023

Published online: 31 Oct 2023 *

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