Title: Isolation and characterisation of copper resistant bacteria from Khetri copper mines and analysis of the expression of copper-induced proteins

Authors: Shraddha Mishra; Sanjay Kumar Verma

Addresses: Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Pilani Campus 333031, Rajasthan, India ' Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, Pilani Campus 333031, Rajasthan, India

Abstract: The present study focuses on the isolation and characterisation of copper-resistant bacteria from Khetri copper mines and analysis of proteins expression under copper stress in selected isolate (KH-5) using SDS-PAGE analysis. A total of 14 different bacterial colonies (KH-1 to KH-14) were isolated on media containing 2 mM of copper and were further characterised for their biochemical properties. The cross-metal tolerance study exhibited their tolerance to other heavy metals (As, Zn, Ni, Co, and Cd) along with copper. The growth curve analysis of all the isolates showed a delay in the lag phase for KH-11, KH-12, KH-2, KH-3, KH-8, and KH-9 in comparison to other strains that indicate the more robust metal resistance mechanisms in other isolates. Based on the results of all these studies, KH-5 was selected for the study of protein expression in the presence of copper stress which showed the same protein band pattern as control (non-stressed condition) without induction of any new protein band in the stressed condition. This suggests the presence of a constitutive copper resistance mechanism in the KH-5. Thus, further studies can be done to explore the copper resistance mechanism in this isolate.

Keywords: copper; tolerance; protein expression.

DOI: 10.1504/IJBET.2023.129816

International Journal of Biomedical Engineering and Technology, 2023 Vol.41 No.3, pp.221 - 230

Received: 12 May 2020
Accepted: 25 Mar 2021
Published online: 31 Mar 2023 *

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