Title: Monitoring optical responses and physiological status of human skin in vivo with diffuse reflectance difference spectroscopy

Authors: Jung Y. Huang; Jyun Ying Chen

Addresses: The T.K.B. Research Center for Photonics, Chiao Tung University, Hsinchu 300, Taiwan ' Department of Photonics, Chiao Tung University, Hsinchu 300, Taiwan

Abstract: Fourier-transform visible-near infrared spectroscopy was applied to analyse diffuse reflectance from human skin perturbed with three skin-agitating methods. Principal component analysis (PCA) was applied to deduce three characteristic spectral responses of human skin. Based on Monte-Carlo multilayer simulation, the responses can be attributed to changes in light scattering and haemoglobin and melanin content. The eigenspectra form a basis for resolving the optical responses of human skin from diffuse reflectance difference spectra measured at different time points after the skin tissue is mechanically stressed. We demonstrate that by applying this analysis scheme on in vivo measured diffuse reflectance difference spectra, valuable information about the responses of skin tissue can be deduced and thereby the physiological status of skin can be monitored.

Keywords: diffuse reflectance spectroscopy; DRS; skin tissue; optical response; Monte-Carlo simulation; principal component analysis; PCA.

DOI: 10.1504/IJBET.2023.129814

International Journal of Biomedical Engineering and Technology, 2023 Vol.41 No.3, pp.300 - 316

Received: 10 Nov 2018
Accepted: 04 Apr 2019

Published online: 31 Mar 2023 *

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